BRENDA - Enzyme Database

Nucleotides of tRNA(Glu) involved in recognition by barley chloroplast glutamyl-tRNA synthetase and glutamyl-tRNA reductase

Willows, R.D.; Kannangara, C.G.; Pontoppidan, B.; Biochim. Biophys. Acta 1263, 228-234 (1995)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.70
chloroplast
-
Hordeum vulgare
9507
-
6.1.1.17
chloroplast
-
Chlamydomonas reinhardtii
9507
-
6.1.1.17
chloroplast
-
Hordeum vulgare
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Chlamydomonas reinhardtii
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Hordeum vulgare
-
-
-
6.1.1.17
Chlamydomonas reinhardtii
-
-
-
6.1.1.17
Hordeum vulgare
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
A7-U66, U29-A41, A53-U61 and U72 are expected to be required for recognition by the barley chloroplast glutamyl-tRNA(Glu) reductase
203
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
6.1.1.17
ATP + glutamate + chloroplastic tRNAGln
misacylation
203
Chlamydomonas reinhardtii
AMP + diphosphate + gluamyl-tRNAGln
-
-
-
-
6.1.1.17
ATP + glutamate + chloroplastic tRNAGln
misacylation
203
Hordeum vulgare
AMP + diphosphate + gluamyl-tRNAGln
-
-
-
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
203
Chlamydomonas reinhardtii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
203
Chlamydomonas reinhardtii
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
chemically modified tRNAGlu modified by monobromobimane or CNBr is a poor substrate. tRNAGlu from the chloroplast of barley, Chlamydomonas reinhardtii, tobacco, cucumber, wheat, and spinach, and tRNAGlu from Synechocystis PCC6803, Escherichia coli, barley germ and bakers yeast are effective substrates, G10, A26, U35 and A37 are recognition elements of barley enzyme
203
Hordeum vulgare
AMP + diphosphate + L-glutamyl-tRNAGlu
-
203
Hordeum vulgare
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
203
Chlamydomonas reinhardtii
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Hordeum vulgare
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Hordeum vulgare
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.70
chloroplast
-
Hordeum vulgare
9507
-
6.1.1.17
chloroplast
-
Chlamydomonas reinhardtii
9507
-
6.1.1.17
chloroplast
-
Hordeum vulgare
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Chlamydomonas reinhardtii
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
A7-U66, U29-A41, A53-U61 and U72 are expected to be required for recognition by the barley chloroplast glutamyl-tRNA(Glu) reductase
203
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
6.1.1.17
ATP + glutamate + chloroplastic tRNAGln
misacylation
203
Chlamydomonas reinhardtii
AMP + diphosphate + gluamyl-tRNAGln
-
-
-
-
6.1.1.17
ATP + glutamate + chloroplastic tRNAGln
misacylation
203
Hordeum vulgare
AMP + diphosphate + gluamyl-tRNAGln
-
-
-
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
203
Chlamydomonas reinhardtii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
203
Chlamydomonas reinhardtii
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
chemically modified tRNAGlu modified by monobromobimane or CNBr is a poor substrate. tRNAGlu from the chloroplast of barley, Chlamydomonas reinhardtii, tobacco, cucumber, wheat, and spinach, and tRNAGlu from Synechocystis PCC6803, Escherichia coli, barley germ and bakers yeast are effective substrates, G10, A26, U35 and A37 are recognition elements of barley enzyme
203
Hordeum vulgare
AMP + diphosphate + L-glutamyl-tRNAGlu
-
203
Hordeum vulgare
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
203
Chlamydomonas reinhardtii
?
-
-
-
-