BRENDA - Enzyme Database

Higher specific activity of the Escherichia coli glutamyl-tRNA synthetase purified to homogeneity by a six-hour procedure

Lin, S.X.; Brisson, A.; Liu, J.; Roy, P.H.; Lapointe, J.; Protein Expr. Purif. 3, 71-74 (1992)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
54000
-
x * 54000, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
overproducing strain DH5alpha(pLQ7612)
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
six-h procedure
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
1.95
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
6.1.1.17
-20°C or -70°C, 20 mM sodium Hepes, pH 7.2, 0.1 mM EDTA, 0.5 mM DTT, 65% glycerol, stable for at least 1 year
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
202
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
202
Escherichia coli
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
?
x * 54000, SDS-PAGE
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
54000
-
x * 54000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
six-h procedure
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
1.95
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.1.1.17
-20°C or -70°C, 20 mM sodium Hepes, pH 7.2, 0.1 mM EDTA, 0.5 mM DTT, 65% glycerol, stable for at least 1 year
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
202
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
202
Escherichia coli
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
?
x * 54000, SDS-PAGE
Escherichia coli