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Literature summary extracted from

  • Inagaki, K.; Tanizawa, K.; Badet, B.; Walsh, C.T.; Tanaka, H.; Soda, K.
    Thermostable alanine racemase from Bacillus stearothermophilus: molecular cloning of the gene, enzyme purification, and characterization (1986), Biochemistry, 25, 3268-3274.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 expression in Escherichia coli C600 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 2.7
-
 L-Ala D-Ala Geobacillus stearothermophilus
5.1.1.1 4.25
-
 L-Ala
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 39000
-
 2 * 39000, SDS-PAGE Geobacillus stearothermophilus
5.1.1.1 78000
-
 equilibrium sedimentation method Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-Ala specific for Ala Geobacillus stearothermophilus D-Ala
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.1 dimer 2 * 39000, SDS-PAGE Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate 2 mol of pyridoxal 5'-phosphate bound per mol of enzyme dimer Geobacillus stearothermophilus