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Literature summary extracted from

  • Badet, B.; Walsh, C.
    Purification of an alanine racemase from Streptococcus faecalis and analysis of its inactivation by (1-aminoethyl)phosphonic acid enantiomers (1985), Biochemistry, 24, 1333-1341.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 (1-Aminoethyl)phosphonate D- and L-(1-aminoethyl)phosphonate Enterococcus faecalis
5.1.1.1 O-Carbamoyl-D-Ser inhibition of wild type enzyme but not of the O-carbamoyl-D-Ser mutant Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 2.2
-
 D-Ala
-
 Enterococcus faecalis
5.1.1.1 7.8
-
 L-Ala
-
 Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 42000
-
 x * 42000, SDS-PAGE Enterococcus faecalis
5.1.1.1 67000
-
 gel filtration Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Enterococcus faecalis
-
 O-carbamoyl-D-Ser-resistant mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1
-
 Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.1 2920
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-Ala
-
 Enterococcus faecalis D-Ala
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.1 ? x * 42000, SDS-PAGE Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.1 9
-
-
 Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate 1 pyridoxal 5'-phosphate per 42000 MW subunit Enterococcus faecalis