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Literature summary extracted from

  • Last, J.A.; Anderson, W.F.
    Purification and properties of bacteriophage T4-induced RNA ligase (1976), Arch. Biochem. Biophys., 174, 167-176.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.3 45000
-
 1 * 45000, SDS-PAGE after reduction with 2-mercaptoethanol Tequatrovirus T4
6.5.1.3 47000 48200 gel filtration, high-speed sedimentation equilibrium centrifugation Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Tequatrovirus T4
-
-
-

Storage Stability

EC Number Storage Stability Organism
6.5.1.3 in liquid nitrogen, at 0.5 mg protein per ml, stable for at least 6 months Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m formation of a phosphodiester bond between a 5'-PO4-terminated oligonucleotide and a 3'-OH terminated oligonucleotide. Intramolecular formation of single-stranded circles with longer oligonucleotides of the type pAp(Ap)nAOH, where n is about 15 or greater. Intermolecular joining of pAp(Ap)3AOH, where the 5'-PO4-terminated oligonucleotide is short enough to prevent apposition of its 3' and 5' ends, to UpUpUOH Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.3 monomer 1 * 45000, SDS-PAGE after reduction with 2-mercaptoethanol Tequatrovirus T4