Literature summary extracted from
Zimmerman, S.B.; Pheiffer, B.H.
Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli (1983), Proc. Natl. Acad. Sci. USA, 80, 5852-5856.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
6.5.1.2 |
Macromolecular solutes |
blunt-end ligation in presence of high concentrations of macromolecular solutes, inactive in absence, e.g. polyethylene glycol 6000, bovine plasma albumin, or Ficoll 70 |
Escherichia coli |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.5.1.2 |
Escherichia coli |
- |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.5.1.2 |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
blunt-end ligation in presence of macromolecular solutes, inactive in absence |
Escherichia coli |
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m |
- |
? |
|
6.5.1.2 |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
PvuII endonuclease-digested pBR322 DNA, formation of linear oligomers ranging from dimer through pentamer |
Escherichia coli |
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m |
- |
? |
|
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
6.5.1.2 |
20 |
37 |
activity at 20°C is lower than at 37°C |
Escherichia coli |