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Literature summary extracted from

  • Zimmerman, S.B.; Pheiffer, B.H.
    Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli (1983), Proc. Natl. Acad. Sci. USA, 80, 5852-5856.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.5.1.2 Macromolecular solutes blunt-end ligation in presence of high concentrations of macromolecular solutes, inactive in absence, e.g. polyethylene glycol 6000, bovine plasma albumin, or Ficoll 70 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Escherichia coli
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m blunt-end ligation in presence of macromolecular solutes, inactive in absence Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m PvuII endonuclease-digested pBR322 DNA, formation of linear oligomers ranging from dimer through pentamer Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.2 20 37 activity at 20°C is lower than at 37°C Escherichia coli