EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.2 | Na+ | above 20-150 mM | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.5.1.2 | 0.0000185 | - |
NAD+ | - |
Thermus thermophilus | |
6.5.1.2 | 0.000025 | - |
5'-phosphoryl termini of poly(dA-dT) | - |
Thermus thermophilus | |
6.5.1.2 | 0.14 | - |
(dT)10 | - |
Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.2 | K+ | activates at 1-150 mM | Thermus thermophilus | |
6.5.1.2 | Mg2+ | requires divalent cations, Mn2+ or Mg2+ | Thermus thermophilus | |
6.5.1.2 | Mg2+ | optimal concentration: 5-9 mM | Thermus thermophilus | |
6.5.1.2 | Mn2+ | requires divalent cations, Mn2+ or Mg2+ | Thermus thermophilus | |
6.5.1.2 | Mn2+ | optimal concentration: 3-6 mM | Thermus thermophilus | |
6.5.1.2 | NH4+ | activates at 1-150 mM | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | 78500 | - |
1 * 78500, SDS-PAGE | Thermus thermophilus |
6.5.1.2 | 80400 | - |
sedimentation equilibrium ultracentrifugation | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.5.1.2 | Thermus thermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.5.1.2 | - |
Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | additional information | - |
- |
Thermus thermophilus |
EC Number | Storage Stability | Organism |
---|---|---|
6.5.1.2 | 4°C, stable for several months | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.2 | ATP + (dT)10 | - |
Thermus thermophilus | ? | - |
? | |
6.5.1.2 | ATP + 5'-phosphoryl termini of poly(dA-dT) | - |
Thermus thermophilus | ? | - |
? | |
6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | (dT)8, (dT)10, (dT)12-18 | Thermus thermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | cohesive-end DNA restriction fragments | Thermus thermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | nick-closing activity | Thermus thermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.5.1.2 | monomer | 1 * 78500, SDS-PAGE | Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | 24 | 37 | cohesive-end DNA restriction fragments | Thermus thermophilus |
6.5.1.2 | 27.7 | - |
p(dT)8, joining of thymidylate oligomers in the presence of poly(dA) as a template | Thermus thermophilus |
6.5.1.2 | 34.5 | - |
p(dT)10, joining of thymidylate oligomers in the presence of poly(dA) as a template | Thermus thermophilus |
6.5.1.2 | 37 | - |
p(dT)12-18, joining of thymidylate oligomers in the presence of poly(dA) as a template | Thermus thermophilus |
6.5.1.2 | 65 | 72 | nick-closing activity | Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | 15 | 85 | nick-closing activity observed in the range | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | 4 | - |
activity decreases remarkably below 4°C | Thermus thermophilus |
6.5.1.2 | 24 | - |
stable for 1 week | Thermus thermophilus |
6.5.1.2 | 37 | - |
stable for 1 week | Thermus thermophilus |
6.5.1.2 | 65 | - |
2 days, 50% loss of activity | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | 7.4 | 7.6 | Tris-HCl buffer | Thermus thermophilus |
6.5.1.2 | 8 | - |
collidine/HCl buffer | Thermus thermophilus |