EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.5.1.2 | polyamines | blunt-end ligation are linear oligomers. Optimal concentration: caldopentamine, 0.05 mM, thermine, 0.1-0.2 mM, spermine, 0.2 mM, thermospermine, 0.4 mM, sperminediol, 0.75 mM | Thermus thermophilus | |
6.5.1.2 | Polyethylene glycol | in presence of high molecular weight polyethylene glycol 20000, 6000 or 1000, 8-28%, the enzyme catalyzes blunt-end intermolecular joining to yield linear oligomers, but no circular DNA forms. In presence of low molecular polyethylene glycols 400, 200, 8-80%, or the monomer ethylene glycol, 16-80%, the circular forms formed by intramolecular ligation are also detected | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.5.1.2 | Thermus thermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | blunt-end ligation, intermolecular joining to yield linear oligomers, but no circular DNA forms in presence of high concentrations of polyethylene glycols | Thermus thermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | 65 | - |
blunt-end ligation | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.5.1.2 | 72 | - |
2 h, stable below, destabilized above | Thermus thermophilus |