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Literature summary extracted from

  • Elder, R.H.; Rossignol, J.M.
    DNA ligases from rat liver. Purification and partial characterization of two molecular forms (1990), Biochemistry, 29, 6009-6017.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 additional information antibodies raised against the 130000 MW polypeptide of DNA ligase I specifically recognize this species in an immunoblot and inhibit only the activity of DNA ligase I Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.012
-
 ATP DNA ligase II Rattus norvegicus
6.5.1.1 0.06
-
 ATP DNA ligase I Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ required Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 100000
-
 x * 100000, DNA ligase II, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Rattus norvegicus
-
 DNA ligase I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 DNA ligase I and II Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase II can use oligo(dT)*poly(rA) as substrate, DNA ligase I not Rattus norvegicus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATPalphaS + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase II can use ATPalphaS much more efficiently than DNA ligase I Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 monomer x * 100000, DNA ligase II, SDS-PAGE Rattus norvegicus