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Literature summary extracted from

  • Doherty, A.J.; Ashford, S.R.; Subramanya, H.S.; Wigley, D.B.
    Bacteriophage T7 DNA ligase. Overexpression, purification, crystallization, and characterization (1996), J. Biol. Chem., 271, 11083-11089.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1
-
 Escherichia phage T7

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.1
-
 Escherichia phage T7

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 AMPPNP nonhydrolyzable ATP analogue Escherichia phage T7
6.5.1.1 AMPPNP no inhibition Tequatrovirus T4
6.5.1.1 EDTA
-
 Escherichia phage T7

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.0003
-
 ATP ATP-diphosphate exchange reaction Escherichia phage T7
6.5.1.1 0.006
-
 ATP dATP Escherichia phage T7
6.5.1.1 0.006
-
 ATP joinig reaction Escherichia phage T7

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 41000
-
 equilibrium sedimentation Escherichia phage T7

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Escherichia phage T7
-
-
-
6.5.1.1 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Escherichia phage T7

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m can ligate nicked, cohesive, and blunt-ended DNA fragments Escherichia phage T7 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 additional information ATP-diphosphate exchange reaction Tequatrovirus T4 ?
-
 ?
6.5.1.1 additional information ATP-diphosphate exchange reaction Escherichia phage T7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 monomer
-
 Tequatrovirus T4
6.5.1.1 monomer 1 * 41000, SDS-PAGE Escherichia phage T7