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Literature summary extracted from

  • Cheng, C.; Shuman, S.
    Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae (1997), Nucleic Acids Res., 25, 1369-1374.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expression in Escherichia coli BL21 Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.0002 0.0015 ATP ATP Haemophilus influenzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ Mn2+ is more effective than Mg2+ at 0.5-1 mM Haemophilus influenzae
6.5.1.1 Mg2+ Mn2+ or Mn2+ required Haemophilus influenzae
6.5.1.1 Mn2+ Mg2+ or Mn2+ required Haemophilus influenzae
6.5.1.1 Mn2+ Mn2+ is more effective than Mg2+ at 0.5-1 mM Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Haemophilus influenzae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.1 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate initial step in DNA ligation is the formation of a covalent enzyme-adenylate intermediate Haemophilus influenzae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m efficient strand joining on a single nicked DNA Haemophilus influenzae AMP + diphosphate + (deoxyribonucleotide)n+m
-
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