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Literature summary extracted from
- Kinetic studies on the reaction catalyzed by DNA ligase from calf thymus (1983), Biochim. Biophys. Acta, 747, 117-122.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | spermidine | 5 mM, increases the Km for nicked DNA, increases reaction velocity | Bos taurus |  |
| 6.5.1.1 | spermine | 0.5 mM, increases the Km for nicked DNA, increases reaction velocity | Bos taurus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | dATP | competitive with respect to ATP | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.5.1.1 | 0.00011 | - |
DNA | nicked DNA 5'-phosphoryl ends | Bos taurus | |
| 6.5.1.1 | 0.002 | - |
ATP | ATP | Bos taurus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | Mg2+ | required | Bos taurus | |
| 6.5.1.1 | Mg2+ | optimal concentration: 10 mM | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.5.1.1 | Bos taurus | - |
calf | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 6.5.1.1 | ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate | uni-uni uni-bi ping-pong mechanism. The order of substrate addition and product release is as follows: ATP, diphosphate, nicked DNA, sealed DNA, 5'-AMP | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 6.5.1.1 | thymus | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.5.1.1 | ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Bos taurus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
| 6.5.1.1 | ATP + DNA | - |
Bos taurus | AMP + diphosphate + ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.5.1.1 | 0.117 | - |
DNA | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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