Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Söderhäll, S.; Lindahl, T.
    Two DNA ligase activities from calf thymus (1973), Biochem. Biophys. Res. Commun., 53, 910-916.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ required Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 85000
-
 and a higher MW form of MW 175000, gel filtration, sucrose density gradient centrifugation Bos taurus
6.5.1.1 175000
-
 DNA ligase I, gel filtration, sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Bos taurus
-
 calf
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m converts nicked circular DNA molecules to a covalently closed form Bos taurus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.1 40
-
 DNA ligase II, rapid loss of activity above Bos taurus
6.5.1.1 42
-
 DNA ligase II, 5 min, 50% inactivation Bos taurus
6.5.1.1 50
-
 DNA ligase I is stable to prolonged heating Bos taurus
6.5.1.1 52
-
 DNA ligase I, 5 min, 50% inactivation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7.4 8 DNA ligase I, in Tris-HCl buffer Bos taurus
6.5.1.1 7.8
-
 DNA ligase II Bos taurus