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Literature summary extracted from

  • Pedrali Noy, G.C.F.; Spadari, S; Ciarrocchi, G.; Pedrini, A.M.; Falaschi, A.
    Two forms of the DNA ligase of human cells (1973), Eur. J. Biochem., 39, 343-351.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ required Homo sapiens
6.5.1.1 Mg2+ Mn2+ and Ca2+ cannot replace Mg2+ in activation Homo sapiens
6.5.1.1 Mn2+ Mn2+ cannot replace Mg2+ in activation Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 95000
-
 DNA ligase II, gel filtration Homo sapiens
6.5.1.1 190000
-
 and a lower MW form of MW 95000, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 EUE cell heteroploid Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
6.5.1.1 -20°C, 50% glycerol w/w, 3 mg/ml bovine serum albumin, stable for up to 7 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase can reconstitute the transforming activity of Bacillus subtilis DNA inactivated by pancreatic DNAse, unable to use a hybrid substrate where an interrupted polydeoxynucleotide is annealed to a polyribonucleotide Homo sapiens AMP + diphosphate + (deoxyribonucleotide)n+m
-
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