Any feedback?
Literature summary extracted from
- Purification and properties of a DNA ligase from a soluble DNA replication complex (1980), Biochim. Biophys. Acta, 606, 202-213.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | dithiothreitol | required for optimal activity | Homo sapiens |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | actinomycin | - |
Homo sapiens | |
| 6.5.1.1 | Ca2+ | CaCl2 inhibits Mg2+-catalyzed reaction | Homo sapiens | |
| 6.5.1.1 | diphosphate | - |
Homo sapiens | |
| 6.5.1.1 | Ethidium bromide | - |
Homo sapiens | |
| 6.5.1.1 | Mn2+ | MnCl2 inhibits Mg2+-catalyzed reaction | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.5.1.1 | 0.0016 | - |
ATP | DNA terminal phosphate | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | K+ | 3fold stimulation at 70 mM KCl, 4fold stimulation at 150 mM KCl | Homo sapiens | |
| 6.5.1.1 | Na+ | KCl is required for optimal activity, 3fold stimulation at 30 mM NaCl, 4fold stimulation at 150 mM NaCl | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 105000 | - |
glycerol density gradient sedimentation | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.5.1.1 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.5.1.1 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 6.5.1.1 | KB cell | adenovirus type 2-infected | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.5.1.1 | ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Homo sapiens | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 25 | 30 | - |
Homo sapiens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 20 | 37 | 20°C: 55% of maximal activity, 37°C: about 70% of maximal activity | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 7.8 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 7 | 8.5 | approximately 70% of maximal activity at pH 7.0 and 8.5 | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
