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Literature summary extracted from

  • Terakoa, H.; Tsukada, K.
    Eukaryotic DNA ligase. Purification and properties of the enzyme from bovine thymus and immunochemical studies of the enzyme from animal tissues (1982), J. Biol. Chem., 257, 4758-4763.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.5.1.1 polyamines polyamines activate at suboptimal concentrations of Mg2+ Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ required Bos taurus
6.5.1.1 Mg2+ Km: 0.9 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 120000 130000 sucrose density gradient centrifugation, highly asymetric shape Bos taurus
6.5.1.1 130000
-
 1 * 130000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.1 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
6.5.1.1 -70°C, stable for at least 2 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Bos taurus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 ? 1 * 130000, SDS-PAGE Bos taurus