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Literature summary extracted from

  • Oishi, N.; Shimada, H.
    Purification and properties of a DNA ligase from sea urchin embryos (1984), J. Biochem., 95, 1187-1192.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 NEM concentrations higher than 1 mM Hemicentrotus pulcherrimus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.004
-
 dATP ATP Hemicentrotus pulcherrimus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ Km: 2.7 mM Hemicentrotus pulcherrimus
6.5.1.1 Mg2+ Mn2+ or Mn2+ required Hemicentrotus pulcherrimus
6.5.1.1 Mn2+ Mg2+ or Mn2+ required Hemicentrotus pulcherrimus
6.5.1.1 Mn2+ Km: 0.3 mM Hemicentrotus pulcherrimus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 80000
-
 gel filtration Hemicentrotus pulcherrimus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Hemicentrotus pulcherrimus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Hemicentrotus pulcherrimus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.1 additional information
-
-
 Hemicentrotus pulcherrimus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Hemicentrotus pulcherrimus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Hemicentrotus pulcherrimus dAMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7.5
-
-
 Hemicentrotus pulcherrimus