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Literature summary extracted from

  • Hayashi, K.; Nakazawa, M.; Ishizaki, Y.; Obayashi, A.
    Influence of monovalent cations on the activity of T4 DNA ligase in the presence of polyethylene glycol (1985), Nucleic Acids Res., 13, 3261-3271.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.5.1.1 Polyethylene glycol polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 K+ extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate Tequatrovirus T4
6.5.1.1 Na+ extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate. Resistance to inhibition in the order from strong to weak: HindIII, PstI, EcoRI, BamHI, SalI in cohesive end ligation, and EcoRV, ScaI, PvuII, NruI in blunt end ligation Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 K+ polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4
6.5.1.1 Na+ polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Tequatrovirus T4
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation is increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intamolecular ligation occurs at low salt concentrations, 0.100 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 10 16 when polyethylene glycol is not present Tequatrovirus T4
6.5.1.1 37
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 with 10% polyethylene glycol 6000 intermolecular ligation at high concentrations of salt is stimulated by raising the temperature from 19°C to 37°C Tequatrovirus T4