EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.4 | (E)-2-methyl-2-butenoyl-CoA | weak | Zea mays | |
6.4.1.4 | acetoacetyl-CoA | - |
Zea mays | |
6.4.1.4 | ATP | - |
Zea mays | |
6.4.1.4 | butanoyl-CoA | weak | Zea mays | |
6.4.1.4 | isobutanoyl-CoA | weak | Zea mays | |
6.4.1.4 | Li+ | slight inhibition | Zea mays | |
6.4.1.4 | n-hexanoyl-CoA | weak | Zea mays | |
6.4.1.4 | Na+ | slight inhibition | Zea mays | |
6.4.1.4 | p-hydroxymercuribenzoate | - |
Zea mays | |
6.4.1.4 | pentanoyl-CoA | weak | Zea mays | |
6.4.1.4 | Phenylglyoxal | 3-methylcrotonoyl-CoA or ATP protects | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.4 | 0.011 | - |
3-methylcrotonoyl-CoA | - |
Zea mays | |
6.4.1.4 | 0.02 | - |
ATP | - |
Zea mays | |
6.4.1.4 | 0.11 | - |
crotonoyl-CoA | - |
Zea mays | |
6.4.1.4 | 0.8 | - |
HCO3- | - |
Zea mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.4 | Co2+ | activates with 57% of the efficiency relative to Mg2+ | Zea mays | |
6.4.1.4 | Cs+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+ | Zea mays | |
6.4.1.4 | K+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+ | Zea mays | |
6.4.1.4 | Mg2+ | maximal activity at 2 mM Mg2+, at ATP concentrations 0.025 mM, 0.05 mM or 1 mM | Zea mays | |
6.4.1.4 | Mg2+ | divalent cation required, Mg2+ is the best activator | Zea mays | |
6.4.1.4 | Mn2+ | activates with 77% of the efficiency relative to Mg2+ | Zea mays | |
6.4.1.4 | NH4+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+ | Zea mays | |
6.4.1.4 | Rb+ | monovalent cations activate, K+, Cs+, Rb+ or NH4+. Maximal activity obtained with Rb+ | Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 58500 | - |
6 * 58500 + 6 * 80000, SDS-PAGE | Zea mays |
6.4.1.4 | 80000 | - |
6 * 58500 + 6 * 80000, SDS-PAGE | Zea mays |
6.4.1.4 | 835000 | - |
PAGE, gel filtration | Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.4 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.4 | - |
Zea mays |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.4.1.4 | ATP + 3-methylcrotonoyl-CoA + HCO3- + H+ = ADP + phosphate + 3-methylglutaconyl-CoA | ATP and HCO3- bind sequentially to the enzyme. ATP and 3-methylcrotonoyl-CoA bind in ping-pong fashion | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.4.1.4 | leaf | - |
Zea mays | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 0.206 | - |
- |
Zea mays |
EC Number | Storage Stability | Organism |
---|---|---|
6.4.1.4 | -20°C, for at least 3 months without loss of activity | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.4 | ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Zea mays | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
6.4.1.4 | ATP + acetoacetyl-CoA + HCO3- | acetoacetyl-CoA inhibits, no activity as substrate | Zea mays | ? | - |
? | |
6.4.1.4 | ATP + crotonoyl-CoA + HCO3- | - |
Zea mays | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.4 | dodecamer | 6 * 58500 + 6 * 80000, SDS-PAGE | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 8 | - |
- |
Zea mays |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.4 | 6.8 | 10.1 | 6.8: more than 70% of maximal activity, 9.5: more than 70% of maximal activity, 10.1: 33% of maximal activity | Zea mays |