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Literature summary extracted from

  • Baldet, P.; Alban, C.; Axiotis, S.; Douce, R.
    Characterization of biotin and 3-methylcrotonyl-coenzyme A carboxylase in higher plant mitochondria (1992), Plant Physiol., 99, 450-455.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.4 Mg2+ above 8 mM Pisum sativum
6.4.1.4 streptavidin
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.4 0.05
-
 3-methylcrotonoyl-CoA
-
 Pisum sativum
6.4.1.4 0.16
-
 ATP
-
 Pisum sativum
6.4.1.4 2.2
-
 HCO3-
-
 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.4.1.4 mitochondrial matrix soluble matrix Pisum sativum 5759
-
6.4.1.4 mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.4 Mg2+ divalent metal ion required, maximal activity at 6 mM Mg2+ Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.4 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.4
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.4 leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.4 ATP + 3-methylcrotonoyl-CoA + HCO3-
-
 Pisum sativum ADP + phosphate + 3-methylglutaconyl-CoA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.4 7.5 9 7.5: about 30% of maximal activity, 9.0: about 85% of maximal activity Pisum sativum
6.4.1.4 8.3
-
-
 Pisum sativum