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Literature summary extracted from

  • Lau, E.P.; Cochran, B.C.; Fall, R.R.
    Isolation of 3-methylcrotonyl-coenzyme A carboxylase from bovine kidney (1980), Arch. Biochem. Biophys., 205, 352-359.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.4 (2E)-3-ethylcrotonoyl-CoA competitive with respect to 3-methylcrotonoyl-CoA Bos taurus
6.4.1.4 (2Z)-geranoyl-CoA competitive with respect to 3-methylcrotonoyl-CoA Bos taurus
6.4.1.4 butanoyl-CoA competitive with respect to 3-methylcrotonoyl-CoA Bos taurus
6.4.1.4 NEM
-
 Bos taurus
6.4.1.4 p-hydroxymercuribenzoate preincubation of the enzyme with ATP or ADP protects the enzyme, while 3-methylcrotonoyl-CoA provides only very slight protection Bos taurus
6.4.1.4 Phenylglyoxal preincubation with ATP or ADP protects, preincubation with 3-methylcrotonoyl-CoA does not Bos taurus
6.4.1.4 propanoyl-CoA competitive with respect to 3-methylcrotonoyl-CoA Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.4 0.022
-
 (2Z)-3-ethylcrotonoyl-CoA
-
 Bos taurus
6.4.1.4 0.075
-
 3-methylcrotonoyl-CoA
-
 Bos taurus
6.4.1.4 0.082
-
 ATP kidney enzyme Bos taurus
6.4.1.4 0.225
-
 (E)-crotonoyl-CoA
-
 Bos taurus
6.4.1.4 1.8
-
 HCO3- kidney enzyme Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.4 Co2+ divalent cation required, Co2+ activates at 34% of the efficiency relative to Mg2+ Bos taurus
6.4.1.4 K+ activates 7fold at 50 mM. Relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ Bos taurus
6.4.1.4 Li+ relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ Bos taurus
6.4.1.4 Mg2+ divalent cation required, Mg2+ is the best activator Bos taurus
6.4.1.4 Mn2+ divalent cation required. Mn2+ activates with 85% of the efficiency relative to Mg2+ Bos taurus
6.4.1.4 Na+ relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ Bos taurus
6.4.1.4 NH4+ relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.4 61000
-
 x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B Bos taurus
6.4.1.4 73500
-
 x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B Bos taurus
6.4.1.4 835000
-
 PAGE, gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.4 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.4 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.4 3.7
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
6.4.1.4 -20°C, 4 months, less than 20% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.4 ATP + (2Z)-3-ethylcrotonoyl-CoA 12% of the activity relative to 3-methylcrotonoyl-CoA Bos taurus ADP + phosphate + 3-ethylglutaconyl-CoA
-
 ?
6.4.1.4 ATP + 3-methylcrotonoyl-CoA + HCO3-
-
 Bos taurus ADP + phosphate + 3-methylglutaconyl-CoA
-
 ?
6.4.1.4 ATP + acetoacetyl-CoA + HCO3- 12% of the activity relative to 3-methylcrotonoyl-CoA Bos taurus ?
-
 ?
6.4.1.4 ATP + crotonoyl-CoA + HCO3- (E)-crotonoyl-CoA Bos taurus ?
-
 ?
6.4.1.4 ATP + crotonoyl-CoA + HCO3- 27% of the activity relative to 3-methylcrotonoyl-CoA Bos taurus ?
-
 ?
6.4.1.4 UTP + 3-methylcrotonoyl-CoA + HCO3- at 62% of the activity relative to ATP Bos taurus UDP + phosphate + 3-methylglutaconyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.4 ? x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.4 38
-
-
 Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.4.1.4 23 50 23°C: 60% of maximal activity, 30°C: 75% of maximal activity, 50°C: 15% of maximal activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.4 8
-
 in presence of Mg2+ and K+ Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.4.1.4 8 9 pH 6.0: no activity, 8.0: maximal activity, pH 9.0: 50% of maximal activity Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.4 biotin enzyme contains biotin Bos taurus
6.4.1.4 biotin contains 1 mol biotin per 157000 MW AB protomeric structure Bos taurus