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Literature summary extracted from

  • Guy, H.I.; Rotgeri, A.; Evans, D.R.
    Activation by fusion of the glutaminase and synthetase subunits of Escherichia coli carbamyl-phosphate synthetase (1997), J. Biol. Chem., 272, 19913-19918.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 additional information the 120000 MW synthetase subunit and the 40000 MW glutaminase subunit, in which the intergenic region between the contiguous carA and carB genes is deleted and the sequence encoding the carbamoyl-phosphate synthase subunits is fused in frame. The resulting fusion protein is activated 10fold relative to the native protein, it is responsive to the allosteric activator Orn, and cannot use NH4+ as a nitrogen donor. The functional linkage that coordinates the rate of glutamine hydrolysis with the activation of bicarbonate is abolished Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 40000
-
 1 * 40000, glutaminase subunit, + 1 * 120000, synthase subunit. The synthase subunit consists of two homologous domains, CPS.A and CPS.B, which catalyze the two different ATP-dependent partial reactions involved in carbamoyl-phosphate synthesis Escherichia coli
6.3.5.5 120000
-
 1 * 40000, glutaminase subunit, + 1 * 120000, synthase subunit. The synthase subunit consists of two homologous domains, CPS.A and CPS.B, which catalyze the two different ATP-dependent partial reactions involved in carbamoyl-phosphate synthesis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 dimer 1 * 40000, glutaminase subunit, + 1 * 120000, synthase subunit. The synthase subunit consists of two homologous domains, CPS.A and CPS.B, which catalyze the two different ATP-dependent partial reactions involved in carbamoyl-phosphate synthesis Escherichia coli