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Literature summary extracted from

  • Yang, H.; Park, S.M.; Nolan, W.G.; Lu, C.D.; Abdelal, A.T.
    Cloning and characterization of the arginine-specific carbamoyl-phosphate synthetase from Bacillus stearothermophilus (1997), Eur. J. Biochem., 249, 443-449.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 5-phospho-alpha-D-ribosyl 1-diphosphate pyrimidine-specific carbamoyl-phosphate synthase is activated, arginine-specific enzyme not Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.5 carbamoyl-phosphate synthase belonging to the pyrimidine pathway Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 Arg arginine-specific carbamoyl-phosphate synthase is repressed, pyrimidine-specific enzyme not Geobacillus stearothermophilus
6.3.5.5 UMP carbamoyl-phosphate synthase belonging to the pyrimidine pathway, carbamoyl-phosphate synthase belonging to the arginine pathway is not inhibited Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 0.18
-
 L-Gln pyrimidine-specific carbamoyl-phosphate synthase Geobacillus stearothermophilus
6.3.5.5 0.6
-
 L-Gln arginine specific carbamoyl-phosphate synthase Geobacillus stearothermophilus
6.3.5.5 9.8
-
 NH4+ carbamoyl-phosphate synthase belonging to the pyrimidine pathway Geobacillus stearothermophilus
6.3.5.5 22
-
 L-Gln carbamoyl-phosphate synthase belonging to the arginine pathway Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 118000
-
 000 + 1 * 118000, carbamoyl-phosphate synthase belonging to the pyrimidine pathway, SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Geobacillus stearothermophilus
-
 2 carbamoyl-phosphate synthases, one specific for pyrimidine biosynthesis and the other for arginine biosynthesis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Geobacillus stearothermophilus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Geobacillus stearothermophilus 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 dimer 000 + 1 * 118000, carbamoyl-phosphate synthase belonging to the pyrimidine pathway, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.5.5 additional information
-
 the thermal stability of cloned enzyme is identical regardless of the growth temperature of Bacillus stearothermophilus between 42°C and 63°C. The thermal stability of the cloned enzyme is not affected by expression at 37°C in Bacillus subtilis or E. coli Geobacillus stearothermophilus