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Literature summary extracted from

  • Abdelal, A.T.; Bussey, L.; Vickers, L.
    Carbamoylphosphate synthetase from Pseudomonas aeruginosa. Subunit composition, kinetic analysis and regulation (1983), Eur. J. Biochem., 129, 697-702.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 acetylornithine activates Pseudomonas aeruginosa
6.3.5.5 Orn activates Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP feedback inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 0.15
-
 Gln
-
 Pseudomonas aeruginosa
6.3.5.5 17
-
 NH4+
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.5 Mg2+ requires free Mg2+, optimal concentration: 4-10 mM Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 12200
-
 1 * 44000 + 1 * 12200, SDS-PAGE Pseudomonas aeruginosa
6.3.5.5 44000
-
 1 * 44000 + 1 * 12200, SDS-PAGE Pseudomonas aeruginosa
6.3.5.5 168000
-
 sucrose density gradient ultracentrifugation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Pseudomonas aeruginosa
-
 derepressed strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.5 2.561
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Pseudomonas aeruginosa 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Pseudomonas aeruginosa 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 dimer 1 * 44000 + 1 * 12200, SDS-PAGE Pseudomonas aeruginosa
6.3.5.5 More self-association does not play a role in regulation of enzyme activity Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.5 8
-
 triethanolamine buffer Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.5 7.5 8.5 7.5: about 65% of maximal activity, 8.5: about 40% of maximal activity Pseudomonas aeruginosa