EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.99.5 | CuSO4 | 2 mM, 20% inhibition | Pseudomonas oleovorans | |
3.5.99.5 | diethyldicarbonate | 2 mM, complete inhibition | Pseudomonas oleovorans | |
3.5.99.5 | MnCl2 | 2 mM, 20% inhibition | Pseudomonas oleovorans | |
3.5.99.5 | additional information | no inhibition by 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid | Pseudomonas oleovorans | |
3.5.99.5 | phenylhydrazine | 10 mM, 20% inhibition | Pseudomonas oleovorans | |
3.5.99.5 | ZnCl2 | 2 mM, 70% inhibition | Pseudomonas oleovorans | |
3.5.99.11 | Cu2+ | 2 mM, 20% inhibition | Pseudomonas oleovorans | |
3.5.99.11 | diethyl dicarbonate | 2 mM, complete loss of activity | Pseudomonas oleovorans | |
3.5.99.11 | Mn2+ | 2 mM, 20% inhibition | Pseudomonas oleovorans | |
3.5.99.11 | additional information | not inhibitory: saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid, or EDTA at 25 mM | Pseudomonas oleovorans | |
3.5.99.11 | phenylhydrazine | 10 mM, 80% inhibition | Pseudomonas oleovorans | |
3.5.99.11 | Zn2+ | 2 mM, 70% inhibition | Pseudomonas oleovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.99.5 | 0.067 | - |
2-aminomuconate | - |
Pseudomonas oleovorans | |
3.5.99.11 | 0.067 | - |
2-aminomuconate | pH 6.6, 25°C | Pseudomonas oleovorans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.99.5 | cytoplasm | - |
Pseudomonas oleovorans | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.99.5 | additional information | no requirement for divalent cations | Pseudomonas oleovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.99.5 | 16600 | - |
6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
3.5.99.5 | 100000 | - |
gel filtration | Pseudomonas oleovorans |
3.5.99.11 | 16600 | - |
6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
3.5.99.11 | 100000 | - |
gel filtration and PAGE | Pseudomonas oleovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.99.5 | 2-aminomuconate + H2O | Pseudomonas oleovorans | intermediate in biodegradation of nitrobenzene | ? | - |
? | |
3.5.99.5 | 2-aminomuconate + H2O | Pseudomonas oleovorans JS45 | intermediate in biodegradation of nitrobenzene | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.99.5 | Pseudomonas oleovorans | - |
JS45 | - |
3.5.99.5 | Pseudomonas oleovorans JS45 | - |
JS45 | - |
3.5.99.11 | Pseudomonas oleovorans | P81593 | - |
- |
3.5.99.11 | Pseudomonas oleovorans JS45 | P81593 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.99.5 | - |
Pseudomonas oleovorans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.99.5 | 37.8 | - |
- |
Pseudomonas oleovorans |
3.5.99.11 | 37.8 | - |
pH 6.6, 25°C | Pseudomonas oleovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.99.5 | 2-aminomuconate + H2O | - |
Pseudomonas oleovorans | 4-oxalocrotonate + NH3 | - |
ir | |
3.5.99.5 | 2-aminomuconate + H2O | - |
Pseudomonas oleovorans JS45 | 4-oxalocrotonate + NH3 | - |
ir | |
3.5.99.5 | 2-aminomuconate + H2O | intermediate in biodegradation of nitrobenzene | Pseudomonas oleovorans | ? | - |
? | |
3.5.99.5 | 2-aminomuconate + H2O | intermediate in biodegradation of nitrobenzene | Pseudomonas oleovorans JS45 | ? | - |
? | |
3.5.99.5 | additional information | no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid | Pseudomonas oleovorans | ? | - |
? | |
3.5.99.5 | additional information | no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid | Pseudomonas oleovorans JS45 | ? | - |
? | |
3.5.99.11 | 2-aminomuconate + H2O | the deamination reaction is irreversible under the assay conditions | Pseudomonas oleovorans | 4-oxalocrotonate + NH3 | enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate | ir | |
3.5.99.11 | 2-aminomuconate + H2O | the deamination reaction is irreversible under the assay conditions | Pseudomonas oleovorans JS45 | 4-oxalocrotonate + NH3 | enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate | ir | |
3.5.99.11 | additional information | no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid | Pseudomonas oleovorans | ? | - |
? | |
3.5.99.11 | additional information | no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid | Pseudomonas oleovorans JS45 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.99.5 | hexamer | 6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
3.5.99.11 | hexamer | 6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.99.5 | 20 | - |
stable for at least 3 days | Pseudomonas oleovorans |
3.5.99.5 | 60 | - |
77% loss of activity in 5 min | Pseudomonas oleovorans |
3.5.99.11 | 22 | - |
stable at room temperature for 3 days | Pseudomonas oleovorans |
3.5.99.11 | 60 | - |
5 min, 23% residual activity | Pseudomonas oleovorans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.99.5 | 208 | - |
2-aminomuconate | - |
Pseudomonas oleovorans | |
3.5.99.11 | 208 | - |
2-aminomuconate | pH 6.6, 25°C | Pseudomonas oleovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.99.5 | 6.6 | - |
- |
Pseudomonas oleovorans |
3.5.99.11 | 6.6 | - |
- |
Pseudomonas oleovorans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.99.5 | 5.7 | 8.8 | at room temperature for at least 3 h | Pseudomonas oleovorans |
3.5.99.11 | 5.7 | 8.8 | room temperature, stable for at least 3 h | Pseudomonas oleovorans |