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Literature summary extracted from

  • Kidder, G.W.; Dewey, V.C.; Nolan, L.L.
    Adenine deaminase of a eukaryotic animal cell, Crithidia fasciculata (1977), Arch. Biochem. Biophys., 183, 7-12.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.4.2 bovine serum albumin protects against heat inactivation in dilute solutions Crithidia fasciculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.2 hypoxanthine competitive, Ki 4 mM Crithidia fasciculata
3.5.4.2 purine 61% at 0.5 mM Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.2 1.5 2 adenine
-
 Crithidia fasciculata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.2 intracellular probably cytosol Crithidia fasciculata 5622
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.2 adenine + H2O Crithidia fasciculata
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.2 Crithidia fasciculata
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.2 0.071
-
 in crude extracts Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.2 adenine + H2O
-
 Crithidia fasciculata hypoxanthine + NH3
-
 ?
3.5.4.2 adenine + H2O
-
 Crithidia fasciculata ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.2 55
-
 less than 10% loss of activity after 60 min Crithidia fasciculata
3.5.4.2 65
-
 20% loss of activity after 5 min Crithidia fasciculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.2 6 7.5
-
 Crithidia fasciculata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.2 5 8.5 pH 5: about 30% of maximal activity, pH 8.5: about 20% of maximal activity Crithidia fasciculata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.4.2 4
-
 hypoxanthine competitive Crithidia fasciculata