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Literature summary extracted from

  • Caperelli, C.A.; Benkovic, P.A.; Chettur, G.; Benkovic, S.J
    Purification of a complex catalizing folate cofactor synthesis and transformylation in de novo purine biosynthesis (1980), J. Biol. Chem., 255, 1885-1890.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 61000
-
 2 * 61000, GAR transformylase, SDS-PAGE Gallus gallus
2.1.2.2 95000
-
 2 * 95000, trifunctional protein, SDS-PAGE Gallus gallus
2.1.2.2 110000
-
 GAR transformylase, sucrose density gradient ultracentrifugation Gallus gallus
2.1.2.2 150000
-
-
 Saccharomyces cerevisiae
2.1.2.2 190000
-
 trifunctional protein, sucrose density gradient ultracentrifugation Gallus gallus
2.1.2.2 201000
-
-
 Ovis aries
2.1.2.2 215000
-
-
 Oryctolagus cuniculus
2.1.2.2 218000
-
-
 Sus scrofa
3.5.4.10 71000
-
 2 * 71000, relative mobility in SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Sus scrofa
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Saccharomyces cerevisiae
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Ovis aries
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Columba sp.
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide Gallus gallus de novo purine synthesis step 10 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Columba sp.
-
 pigeon
-
2.1.2.2 Gallus gallus
-
 chicken
-
2.1.2.2 Oryctolagus cuniculus
-
 rabbit
-
2.1.2.2 Ovis aries
-
 sheep
-
2.1.2.2 Saccharomyces cerevisiae
-
 yeast
-
2.1.2.2 Sus scrofa
-
 pig
-
3.5.4.10 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2
-
 Gallus gallus
2.1.2.2
-
 Sus scrofa
2.1.2.2
-
 Saccharomyces cerevisiae
2.1.2.2
-
 Oryctolagus cuniculus
2.1.2.2
-
 Ovis aries
2.1.2.2
-
 Columba sp.
3.5.4.10 from liver homogenate Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.2 liver
-
 Gallus gallus
-
2.1.2.2 liver
-
 Sus scrofa
-
2.1.2.2 liver
-
 Oryctolagus cuniculus
-
2.1.2.2 liver
-
 Ovis aries
-
2.1.2.2 liver
-
 Columba sp.
-
3.5.4.10 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.2 0.651
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Sus scrofa tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Saccharomyces cerevisiae tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Ovis aries tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Columba sp. tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 additional information purified along with 10-formyltetrahydrofolate synthetase Gallus gallus ?
-
 ?
2.1.2.2 additional information uses both diastereomers of 5,10-methenyltetrahydrofolate, (+)-L-diastereomer is used 120 times faster than the (-)-L-diastereomer Gallus gallus ?
-
 ?
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide de novo purine synthesis step 10 Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.2 dimer
-
 Saccharomyces cerevisiae
2.1.2.2 dimer
-
 Ovis aries
2.1.2.2 dimer 2 * 61000, GAR transformylase, SDS-PAGE Gallus gallus
2.1.2.2 dimer 2 * 95000, trifunctional protein, SDS-PAGE Gallus gallus
3.5.4.10 dimer 2 * 71000, relative mobility in SDS-PAGE Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.2 5,10-methenyltetrahydrofolate
-
 Gallus gallus
2.1.2.2 N10-formyltetrahydrofolate
-
 Gallus gallus