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Literature summary extracted from

  • Vigetti, D.; Monetti, C.; Pollegioni, L.; Taramelli, R.; Bernardini, G.
    Xenopus allantoicase: Molecular cloning, enzymatic activity and developmental expression (2000), Arch. Biochem. Biophys., 379, 90-96.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.4 EDTA 1 mM, more than 200% of initial activity Xenopus laevis
3.5.3.4 EGTA 1 mM, about 200% of initial activity Xenopus laevis
3.5.3.4 additional information no activation by EDTA or EGTA Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.4 expression in Escherichia coli Xenopus laevis
3.5.3.4 expression in Escherichia coli Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.4 Ca2+ 1 mM, about 50% of inhibition, reversed by EDTA Xenopus laevis
3.5.3.4 Mg2+ 1 mM, about 50% of inhibition, reversed by EGTA Xenopus laevis
3.5.3.4 additional information not inhibitory: Ca2+, Mg2+, EDTA, EGTA Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.4 4.5
-
 Allantoate pH 7.0, 25°C Xenopus laevis
3.5.3.4 8.44
-
 Allantoate
-
 Xenopus laevis
3.5.3.4 8.44
-
 Allantoate from kidney Xenopus laevis
3.5.3.4 250
-
 Allantoate pH 7.0, 25°C Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.4 43700
-
 x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE Xenopus laevis
3.5.3.4 44700
-
 x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE Xenopus laevis
3.5.3.4 46500
-
 x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE Mus musculus
3.5.3.4 47200
-
 x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.4 Mus musculus Q9JHX6
-
-
3.5.3.4 Xenopus laevis
-
-
-
3.5.3.4 Xenopus laevis Q9W6S5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.4
-
 Xenopus laevis
3.5.3.4 recombinant enzyme Xenopus laevis
3.5.3.4 recombinant enzyme Mus musculus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.3.4 inhibition by divalent cations is reversed by excess of chaletors of these cations Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.4 kidney
-
 Xenopus laevis
-
3.5.3.4 liver
-
 Xenopus laevis
-
3.5.3.4 lung
-
 Xenopus laevis
-

Storage Stability

EC Number Storage Stability Organism
3.5.3.4 -20°C, 50 mM phosphate buffer, pH 7.0, stable Xenopus laevis
3.5.3.4 -20°C, 50 mM phosphate buffer, pH 7.0, stable Mus musculus
3.5.3.4 4°C, above, rapid loss of activity Xenopus laevis
3.5.3.4 4°C, above, rapid loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.4 (-)-ureidoglycolate
-
 Xenopus laevis glyoxylate + urea
-
 ?
3.5.3.4 allantoate + H2O
-
 Xenopus laevis (-)-ureidoglycolate + urea
-
 r
3.5.3.4 allantoate + H2O
-
 Xenopus laevis (S)-ureidoglycolate + urea
-
 ?
3.5.3.4 allantoate + H2O
-
 Mus musculus (S)-ureidoglycolate + urea
-
 ?
3.5.3.4 allantoate + H2O
-
 Xenopus laevis ureidoglycolate + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.4 ? x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE Xenopus laevis
3.5.3.4 ? x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.5.3.4 allantoicase
-
 Xenopus laevis
3.5.3.4 XAlc
-
 Xenopus laevis
3.5.3.4 XAlc
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.4 40 45
-
 Xenopus laevis
3.5.3.4 50 55
-
 Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.4 40
-
 15 min, loss of about 50% of activity Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.4 7
-
 assay at Xenopus laevis
3.5.3.4 7
-
 assay at Mus musculus