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Literature summary extracted from
- Creatinine hydrolase and creatine amidinohydrolase. II. Partial purification and properties (1974), Mol. Cell. Biochem., 3, 17-25.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.10 | azide | complete inhibition at the ionic strength of 2 mM | Paenarthrobacter ureafaciens |  |
| 3.5.2.10 | Cu2+ | complete inhibition at the ionic strength of 1 mM CuSO4 | Paenarthrobacter ureafaciens | |
| 3.5.2.10 | cyanide | complete inhibition by 1 mM, much less inhibition by both azide and cyanide | Paenarthrobacter ureafaciens | |
| 3.5.2.10 | Hg2+ | complete inhibition at the ionic strength of 0.1 mM HgCl, 20-30% activity restored at 20 mM 2-mercaptoethanol | Paenarthrobacter ureafaciens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.2.10 | 125 | - |
creatinine | - |
Paenarthrobacter ureafaciens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.10 | 240000 | - |
gel filtration | Paenarthrobacter ureafaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.10 | Paenarthrobacter ureafaciens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.2.10 | heating, precipitation of nucleic acids with streptomycin, ammonium sulfate precipitation, Sephadex gel filtration, and DEAE-cellulose column chromatography | Paenarthrobacter ureafaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.10 | 8.3 | - |
- |
Paenarthrobacter ureafaciens |
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Release 2023.1 (January 2023)
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