Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rikitake, K.; Oka, I.; Ando, M.; Yoshimoto, T.; Tsuru, D.
    Creatinine amidohydrolase (creatininase) from Pseudomonas putida. Purification and some properties (1979), J. Biochem., 86, 1109-1117.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.10 Co2+
-
 Pseudomonas putida
3.5.2.10 Cu2+
-
 Pseudomonas putida
3.5.2.10 EDTA
-
 Pseudomonas putida
3.5.2.10 ethoxyformic anhydride
-
 Pseudomonas putida
3.5.2.10 Fe2+
-
 Pseudomonas putida
3.5.2.10 heavy metal ions
-
 Pseudomonas putida
3.5.2.10 Mg2+
-
 Pseudomonas putida
3.5.2.10 additional information
-
 Pseudomonas putida
3.5.2.10 N-bromosuccinimide
-
 Pseudomonas putida
3.5.2.10 o-phenanthroline
-
 Pseudomonas putida
3.5.2.10 PCMB
-
 Pseudomonas putida
3.5.2.10 Photooxidation
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.10 26
-
 creatinine
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.10 Co2+ activates Pseudomonas putida
3.5.2.10 Fe2+ activates Pseudomonas putida
3.5.2.10 Mg2+ activates Pseudomonas putida
3.5.2.10 Mn2+ activates Pseudomonas putida
3.5.2.10 Ni2+ activates Pseudomonas putida
3.5.2.10 Zn2+ one gatom Zn2+ per mol of subunit monomer Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.10 23000
-
 8 * 23000, SDS-PAGE Pseudomonas putida
3.5.2.10 175000
-
 ultracentrifugal analysis Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.10 creatinine + H2O Pseudomonas putida
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.10 Pseudomonas putida
-
 var. naraensis, strain C-83
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.2.10 unstable to photooxidation Pseudomonas putida

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.2.10 glycoprotein 3.5% glucose Pseudomonas putida

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.10 purified by column chromarographies on sarcosine-HM-Sepharose and DEAE-cellulose, and gel filtration on Sephadex G-200 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.10 488
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
3.5.2.10 -20°C, 6 months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.10 creatinine + H2O
-
 Pseudomonas putida creatine
-
 ?
3.5.2.10 creatinine + H2O
-
 Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.10 octamer 8 * 23000, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.10 55
-
 30 min at pH 7.1, apoenzyme Pseudomonas putida
3.5.2.10 70
-
 30 min at pH 7.1, native enzyme Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.10 7 9
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.10 6 12 for 3 h at 30°C Pseudomonas putida