EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.67 | ADP | slight activation | Arachis hypogaea | |
3.5.1.67 | benzalkonium chloride | activation, 126% activity | Arachis hypogaea | |
3.5.1.67 | bovine serum albumin | activation, 0.5%: 180% activity | Arachis hypogaea | |
3.5.1.67 | Detergents | ionic or non-ionic detergents, 3-4fold activation | Arachis hypogaea | |
3.5.1.67 | methionine sulfoximine | very slight activation | Arachis hypogaea | |
3.5.1.67 | additional information | activation studies | Arachis hypogaea | |
3.5.1.67 | additional information | no activation by dithiothreitol | Arachis hypogaea | |
3.5.1.67 | rabbit preimmune serum | activation, 0.5%: 180% activity | Arachis hypogaea | |
3.5.1.67 | SDS | activation | Arachis hypogaea | |
3.5.1.67 | SDS | 0.001-1%, 2-3fold stimulation, 4-methylene-L-glutamine or glutamine as substrate | Arachis hypogaea | |
3.5.1.67 | sodium deoxycholate | activation, 160% activity | Arachis hypogaea | |
3.5.1.67 | sodium octanoate | activation, 130% activity | Arachis hypogaea | |
3.5.1.67 | Triton X-100 | activation, 180% activity | Arachis hypogaea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.67 | 4-methylene-L-glutamate | slight inhibition | Arachis hypogaea | |
3.5.1.67 | 4-methylene-L-glutamine | competitive, glutamine as substrate, Ki: 30 mM | Arachis hypogaea | |
3.5.1.67 | albizziine | 3-ureidoalanine, glutamine analog, competitive, 4 mM: 33% inhibition, 10 mM: 50% inhibition, 20 mM: 68% inhibition | Arachis hypogaea | |
3.5.1.67 | BO33- | strong inhibition, 1 mM: 55% inhibition, 2 mM: 70% inhibition, 5 mM: 87% inhibition | Arachis hypogaea | |
3.5.1.67 | diazo-oxo-norleucine | very slight inhibition, 4 mM: 7% inhibition | Arachis hypogaea | |
3.5.1.67 | L-glutamine | competitive, 20 mM: 50% inhibition, 4-methylene-L-glutamine as substrate | Arachis hypogaea | |
3.5.1.67 | additional information | inhibition studies; no inhibition by methionine sulfoximine, ADP | Arachis hypogaea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.67 | additional information | - |
additional information | kinetic data | Arachis hypogaea | |
3.5.1.67 | 5 | - |
4-methylene-L-glutamine | - |
Arachis hypogaea | |
3.5.1.67 | 20 | - |
L-glutamine | - |
Arachis hypogaea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.67 | phosphate | slight activation | Arachis hypogaea | |
3.5.1.67 | Phosphate esters | slight activation | Arachis hypogaea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.67 | 4-methylene-L-glutamine + H2O | Arachis hypogaea | highly specific | ? | - |
? | |
3.5.1.67 | 4-methylene-L-glutamine + H2O | Arachis hypogaea | implicated in the process of N-translocation | ? | - |
? | |
3.5.1.67 | 4-methylene-L-glutamine + H2O | Arachis hypogaea | key evidence in establishing N-translocating role for this nonprotein amino acid | ? | - |
? | |
3.5.1.67 | additional information | Arachis hypogaea | implicated in the process of N-translocation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.67 | Arachis hypogaea | - |
Valencia, cv Tennessee Red | - |
3.5.1.67 | Arachis hypogaea | - |
two electrophoretically distinct, immunologically identical forms with similar catalytic properties | - |
3.5.1.67 | Arachis hypogaea | - |
multiple electrophoretically distinct forms | - |
3.5.1.67 | Arachis hypogaea | - |
peanut, groundnut | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.67 | multiple electrophoretically distinct forms | Arachis hypogaea |
3.5.1.67 | two catalytically active forms | Arachis hypogaea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.67 | leaf | 14- to 16-day germinated peanuts | Arachis hypogaea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.67 | 349 | - |
- |
Arachis hypogaea |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.67 | 4°C, in solution, stable for many months | Arachis hypogaea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.67 | 4-methylene-L-glutamine + H2O | highly specific | Arachis hypogaea | 4-methylene-L-glutamate + NH3 | - |
? | |
3.5.1.67 | 4-methylene-L-glutamine + H2O | highly specific | Arachis hypogaea | ? | - |
? | |
3.5.1.67 | 4-methylene-L-glutamine + H2O | implicated in the process of N-translocation | Arachis hypogaea | ? | - |
? | |
3.5.1.67 | 4-methylene-L-glutamine + H2O | key evidence in establishing N-translocating role for this nonprotein amino acid | Arachis hypogaea | ? | - |
? | |
3.5.1.67 | L-glutamine + H2O | - |
Arachis hypogaea | L-glutamate + NH3 | - |
? | |
3.5.1.67 | additional information | implicated in the process of N-translocation | Arachis hypogaea | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.67 | More | two electrophoretically distinct catalytically active forms: band I: 57000 and 50000 | Arachis hypogaea |
3.5.1.67 | More | probably a heterodimer, band II: 50000, probably a homodimer, PAGE | Arachis hypogaea |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.67 | 37 | - |
assay at | Arachis hypogaea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.67 | additional information | - |
AH-3: pI: 5.1 | Arachis hypogaea |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.67 | 30 | - |
4-methylene-L-glutamine | competitive, glutamine as substrate | Arachis hypogaea |