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Literature summary extracted from

  • Matsumura, E.; Shin, T.; Murao, S.; Sakaguchi, M.; Kawano, T.
    A novel enzyme, Nalpha-benzyloxycarbonyl amino acid urethane hydrolase IV (1985), Agric. Biol. Chem., 49, 3643-3645.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.64 EDTA 1 mM: complete inhibition Soil bacterium
3.5.1.64 iodoacetate 1 mM: strong inhibition Soil bacterium
3.5.1.64 p-chloromercuribenzoate 1 mM: strong inhibition Soil bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.64 Co2+ required for activity, optimum concentration about 1 mM Soil bacterium
3.5.1.64 divalent cations required for activity Soil bacterium
3.5.1.64 Zn2+ required for activity, optimum concentration about 1 mM Soil bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.64 39000
-
 strain F-96, x * 39000, SDS-PAGE Soil bacterium
3.5.1.64 116000
-
 strain F-96, gel filtration Soil bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.64 Soil bacterium
-
-
-
3.5.1.64 Soil bacterium F-96
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.64 strain F-96 Soil bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.64 additional information substrate specificity Soil bacterium ?
-
 ?
3.5.1.64 additional information hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids Soil bacterium ?
-
 ?
3.5.1.64 additional information broadly selective amino acid specificity Soil bacterium ?
-
 ?
3.5.1.64 additional information specific for L-isomers Soil bacterium ?
-
 ?
3.5.1.64 additional information no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein Soil bacterium ?
-
 ?
3.5.1.64 additional information inactive toward Nalpha-benzyloxycarbonyl-D-amino acids Soil bacterium ?
-
 ?
3.5.1.64 additional information requires substrates with free carboxyl group of amino acids Soil bacterium ?
-
 ?
3.5.1.64 additional information substrate specificity Soil bacterium F-96 ?
-
 ?
3.5.1.64 additional information hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids Soil bacterium F-96 ?
-
 ?
3.5.1.64 additional information broadly selective amino acid specificity Soil bacterium F-96 ?
-
 ?
3.5.1.64 additional information specific for L-isomers Soil bacterium F-96 ?
-
 ?
3.5.1.64 additional information no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein Soil bacterium F-96 ?
-
 ?
3.5.1.64 Nalpha-(4-aminobenzoyl)-L-Glu + H2O poor substrate Soil bacterium 1-amino-4-hydroxybenzene + CO2 + L-Glu
-
 ?
3.5.1.64 Nalpha-(4-hydroxybenzoyl)-L-Asp + H2O poor substrate Soil bacterium 1,4-dihydroxybenzene + CO2 + L-Asp
-
 ?
3.5.1.64 Nalpha-acetyl-L-Asp + H2O poor substrate Soil bacterium methanol + CO2 + L-Asp
-
 ?
3.5.1.64 Nalpha-acetyl-L-Glu + H2O poor substrate Soil bacterium methanol + CO2 + L-Glu
-
 ?
3.5.1.64 Nalpha-acetyl-L-Leu + H2O 40% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis Soil bacterium methanol + CO2 + L-Leu
-
 ?
3.5.1.64 Nalpha-benzoyl-L-Glu + H2O
-
 Soil bacterium phenol + CO2 + L-Glu
-
 ?
3.5.1.64 Nalpha-benzoyl-L-Leu + H2O
-
 Soil bacterium phenol + CO2 + L-Leu
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-Gly + H2O poor substrate Soil bacterium benzyl alcohol + CO2 + Gly
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Ala + H2O
-
 Soil bacterium benzyl alcohol + CO2 + L-Ala
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Asn + H2O poor substrate Soil bacterium benzyl alcohol + CO2 + L-Asn
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Asp + H2O
-
 Soil bacterium benzyl alcohol + CO2 + L-Asp
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Cys + H2O poor substrate Soil bacterium benzyl alcohol + CO2 + L-Cys
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Gln + H2O poor substrate Soil bacterium benzyl alcohol + CO2 + L-Gln
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Glu + H2O
-
 Soil bacterium benzyl alcohol + CO2 + L-Glu
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-His + H2O very poor substrate Soil bacterium benzyl alcohol + CO2 + L-His
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Ile + H2O very poor substrate Soil bacterium benzyl alcohol + CO2 + L-Ile
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Met + H2O
-
 Soil bacterium benzyl alcohol + CO2 + L-Met
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Phe + H2O very poor substrate Soil bacterium benzyl alcohol + CO2 + L-Phe
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Ser + H2O poor substrate Soil bacterium benzyl alcohol + CO2 + L-Ser
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Thr + H2O very poor substrate Soil bacterium benzyl alcohol + CO2 + L-Thr
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Tyr + H2O very poor substrate Soil bacterium benzyl alcohol + CO2 + L-Tyr
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-L-Val + H2O poor substrate Soil bacterium benzyl alcohol + CO2 + L-Val
-
 ?
3.5.1.64 Nalpha-benzyloxycarbonyl-Leu + H2O best substrate Soil bacterium benzyl alcohol + CO2 + L-Leu
-
 ?
3.5.1.64 Nalpha-tert-butoxycarbonyl-Gly + H2O
-
 Soil bacterium tert-butanol + CO2 + Gly
-
 ?
3.5.1.64 Nalpha-tert-butoxycarbonyl-L-Ala + H2O
-
 Soil bacterium tert-butanol + CO2 + L-Ala
-
 ?
3.5.1.64 Nalpha-tert-butoxycarbonyl-L-Asp + H2O
-
 Soil bacterium tert-butanol + CO2 + L-Asp
-
 ?
3.5.1.64 Nalpha-tert-butoxycarbonyl-L-Gln + H2O poor substrate Soil bacterium tert-butanol + CO2 + L-Gln
-
 ?
3.5.1.64 Nalpha-tert-butoxycarbonyl-L-Leu + H2O 80% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis Soil bacterium tert-butanol + CO2 + L-Leu
-
 ?
3.5.1.64 Nalpha-tert-butoxycarbonyl-L-Met + H2O poor substrate Soil bacterium tert-butanol + CO2 + L-Met
-
 ?
3.5.1.64 Nalpha-tert-butoxycarbonyl-L-Val + H2O poor substrate Soil bacterium tert-butanol + CO2 + L-Val
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.64 ? strain F-96, x * 39000, SDS-PAGE Soil bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.64 55
-
-
 Soil bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.64 37
-
 17 h, pH 6-7, stable at Soil bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.64 additional information
-
 pI: 3.31 Soil bacterium
3.5.1.64 5.1
-
-
 Soil bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.64 6 7 17 h, 37°C, stable at Soil bacterium