BRENDA - Enzyme Database

A novel enzyme, Nalpha-benzyloxycarbonyl amino acid urethane hydrolase IV

Matsumura, E.; Shin, T.; Murao, S.; Sakaguchi, M.; Kawano, T.; Agric. Biol. Chem. 49, 3643-3645 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.64
EDTA
1 mM: complete inhibition
Soil bacterium
3.5.1.64
iodoacetate
1 mM: strong inhibition
Soil bacterium
3.5.1.64
p-chloromercuribenzoate
1 mM: strong inhibition
Soil bacterium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.64
Co2+
required for activity, optimum concentration about 1 mM
Soil bacterium
3.5.1.64
divalent cations
required for activity
Soil bacterium
3.5.1.64
Zn2+
required for activity, optimum concentration about 1 mM
Soil bacterium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.64
39000
-
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
3.5.1.64
116000
-
strain F-96, gel filtration
Soil bacterium
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.5.1.64
Soil bacterium
-
-
-
3.5.1.64
Soil bacterium F-96
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.5.1.64
strain F-96
Soil bacterium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.5.1.64
additional information
substrate specificity
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
broadly selective amino acid specificity
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
specific for L-isomers
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
inactive toward Nalpha-benzyloxycarbonyl-D-amino acids
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
requires substrates with free carboxyl group of amino acids
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
substrate specificity
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
broadly selective amino acid specificity
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
specific for L-isomers
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
Nalpha-(4-aminobenzoyl)-L-Glu + H2O
poor substrate
172071
Soil bacterium
1-amino-4-hydroxybenzene + CO2 + L-Glu
-
-
-
?
3.5.1.64
Nalpha-(4-hydroxybenzoyl)-L-Asp + H2O
poor substrate
172071
Soil bacterium
1,4-dihydroxybenzene + CO2 + L-Asp
-
-
-
?
3.5.1.64
Nalpha-acetyl-L-Asp + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Asp
-
-
-
?
3.5.1.64
Nalpha-acetyl-L-Glu + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Glu
-
-
-
?
3.5.1.64
Nalpha-acetyl-L-Leu + H2O
40% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
methanol + CO2 + L-Leu
-
-
-
?
3.5.1.64
Nalpha-benzoyl-L-Glu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Glu
-
-
-
?
3.5.1.64
Nalpha-benzoyl-L-Leu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Leu
-
-
-
?
3.5.1.64
Nalpha-benzyloxycarbonyl-Gly + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + Gly
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ala
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Asn + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asn
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asp
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Cys + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Cys
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Gln
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Glu + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Glu
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-His + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-His
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Ile + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ile
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Met + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Met
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Phe + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Phe
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Ser + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ser
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Thr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Thr
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Tyr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Tyr
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Val
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-Leu + H2O
best substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Leu
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-Gly + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + Gly
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Ala
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Asp
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Gln
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Leu + H2O
80% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
tert-butanol + CO2 + L-Leu
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Met + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Met
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Val
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.64
?
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.64
55
-
-
Soil bacterium
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.1.64
37
-
17 h, pH 6-7, stable at
Soil bacterium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.64
additional information
-
pI: 3.31
Soil bacterium
3.5.1.64
5.1
-
-
Soil bacterium
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.64
6
7
17 h, 37C, stable at
Soil bacterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.64
EDTA
1 mM: complete inhibition
Soil bacterium
3.5.1.64
iodoacetate
1 mM: strong inhibition
Soil bacterium
3.5.1.64
p-chloromercuribenzoate
1 mM: strong inhibition
Soil bacterium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.64
Co2+
required for activity, optimum concentration about 1 mM
Soil bacterium
3.5.1.64
divalent cations
required for activity
Soil bacterium
3.5.1.64
Zn2+
required for activity, optimum concentration about 1 mM
Soil bacterium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.64
39000
-
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
3.5.1.64
116000
-
strain F-96, gel filtration
Soil bacterium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.64
strain F-96
Soil bacterium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.5.1.64
additional information
substrate specificity
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
broadly selective amino acid specificity
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
specific for L-isomers
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
inactive toward Nalpha-benzyloxycarbonyl-D-amino acids
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
requires substrates with free carboxyl group of amino acids
172071
Soil bacterium
?
-
-
-
?
3.5.1.64
additional information
substrate specificity
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
broadly selective amino acid specificity
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
specific for L-isomers
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium F-96
?
-
-
-
?
3.5.1.64
Nalpha-(4-aminobenzoyl)-L-Glu + H2O
poor substrate
172071
Soil bacterium
1-amino-4-hydroxybenzene + CO2 + L-Glu
-
-
-
?
3.5.1.64
Nalpha-(4-hydroxybenzoyl)-L-Asp + H2O
poor substrate
172071
Soil bacterium
1,4-dihydroxybenzene + CO2 + L-Asp
-
-
-
?
3.5.1.64
Nalpha-acetyl-L-Asp + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Asp
-
-
-
?
3.5.1.64
Nalpha-acetyl-L-Glu + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Glu
-
-
-
?
3.5.1.64
Nalpha-acetyl-L-Leu + H2O
40% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
methanol + CO2 + L-Leu
-
-
-
?
3.5.1.64
Nalpha-benzoyl-L-Glu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Glu
-
-
-
?
3.5.1.64
Nalpha-benzoyl-L-Leu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Leu
-
-
-
?
3.5.1.64
Nalpha-benzyloxycarbonyl-Gly + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + Gly
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ala
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Asn + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asn
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asp
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Cys + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Cys
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Gln
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Glu + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Glu
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-His + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-His
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Ile + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ile
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Met + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Met
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Phe + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Phe
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Ser + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ser
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Thr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Thr
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Tyr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Tyr
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Val
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-benzyloxycarbonyl-Leu + H2O
best substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Leu
-
172071
Soil bacterium
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-Gly + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + Gly
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Ala
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Asp
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Gln
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Leu + H2O
80% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
tert-butanol + CO2 + L-Leu
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Met + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Met
-
-
-
?
3.5.1.64
Nalpha-tert-butoxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Val
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.64
?
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.64
55
-
-
Soil bacterium
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.1.64
37
-
17 h, pH 6-7, stable at
Soil bacterium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.64
additional information
-
pI: 3.31
Soil bacterium
3.5.1.64
5.1
-
-
Soil bacterium
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.64
6
7
17 h, 37C, stable at
Soil bacterium