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Literature summary extracted from

  • Wasternack, C.; Lippmann, G.; Reinbotte, H.
    Pyrimidine-degrading enzymes purification and properties of beta-ureidopropionase of Euglena gracilis (1979), Biochim. Biophys. Acta, 570, 341-351.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.6 acetate competitive inhibition Euglena gracilis
3.5.1.6 Alpha-fluoroacetate competitive inhibition Euglena gracilis
3.5.1.6 Beta-ureidoisobutyric acid competitive inhibition Euglena gracilis
3.5.1.6 beta-ureidopropionate competitive inhibition Euglena gracilis
3.5.1.6 Cu2+
-
 Euglena gracilis
3.5.1.6 Isobutyrate competitive inhibition Euglena gracilis
3.5.1.6 propionate competitive inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.6 0.018
-
 Beta-ureidoisobutyric acid
-
 Euglena gracilis
3.5.1.6 0.038
-
 beta-ureidopropionic acid
-
 Euglena gracilis
3.5.1.6 0.5
-
 beta-ureidopropionic acid
-
 Rattus rattus
3.5.1.6 0.63
-
 beta-ureidopropionic acid
-
 Clostridium uracilicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.6 Mg2+
-
 Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.6 1500000 2000000 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.6 N-carbamoyl-beta-alanine + H2O Mus musculus
-
 ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O Euglena gracilis
-
 ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O Rattus rattus
-
 ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O Clostridium uracilicum
-
 ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O Clostridium uracilicum M5-2
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.6 Clostridium uracilicum
-
-
-
3.5.1.6 Clostridium uracilicum M5-2
-
-
-
3.5.1.6 Euglena gracilis
-
 plant-like unicellular flagellate
-
3.5.1.6 Mus musculus
-
-
-
3.5.1.6 Rattus rattus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.6 partially Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.6 liver
-
 Mus musculus
-
3.5.1.6 liver
-
 Rattus rattus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.6 14.7
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Mus musculus beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Euglena gracilis beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Rattus rattus beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Clostridium uracilicum beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Clostridium uracilicum M5-2 beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Mus musculus ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Euglena gracilis ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Rattus rattus ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Clostridium uracilicum ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Clostridium uracilicum M5-2 ?
-
 ?
3.5.1.6 N-carbamoyl-DL-beta-aminoisobutyrate + H2O
-
 Euglena gracilis DL-beta-aminoisobutyrate + CO2 + NH3
-
 ?
3.5.1.6 N-carbamoyl-DL-beta-aminoisobutyrate + H2O
-
 Rattus rattus DL-beta-aminoisobutyrate + CO2 + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.6 60
-
-
 Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.6 6.3
-
-
 Euglena gracilis