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Literature summary extracted from

  • Kinoshita, S.; Terada, T.; Taniguchi, T.; Takene, Y.; Masuda, S.
    Purification and characterization of 6-aminohexanoic acid-oligomer hydrolase of Flavobacterium sp. KI72 (1981), Eur. J. Biochem., 116, 547-551.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.46 diisopropyl fluorophosphate inhibition at 0.37 mM Flavobacterium sp.
3.5.1.46 p-chloromercuribenzoate inhibition at 0.013 mM Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.46 5.9
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Flavobacterium sp.
3.5.1.46 6.2
-
 N-6-aminohexanoate-trimer
-
 Flavobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.46 intracellular
-
 Flavobacterium sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.46 42000
-
 2 * 42000, SDS-PAGE Flavobacterium sp.
3.5.1.46 84000
-
 gel filtration Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.46 Flavobacterium sp.
-
-
-
3.5.1.46 Flavobacterium sp. KI72
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.46 to homogeneity, chromatography techniques, preparative gel electrophoresis Flavobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.46 cell culture
-
 Flavobacterium sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Flavobacterium sp. 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-6-aminohexanoate hexamer + H2O
-
 Flavobacterium sp. 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate hexamer + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate icosamer + H2O poor activity, decreasing hydrolysis with increasing polymerization number of oligomers, higher oligomers not hydrolyzed Flavobacterium sp. 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate pentamer + H2O
-
 Flavobacterium sp. 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate pentamer + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate tetramer + H2O
-
 Flavobacterium sp. 6-aminohexanoate + N-6-aminohexanoate trimer
-
 ?
3.5.1.46 N-6-aminohexanoate tetramer + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + N-6-aminohexanoate trimer
-
 ?
3.5.1.46 N-6-aminohexanoate trimer + H2O
-
 Flavobacterium sp. 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.1.46 N-6-aminohexanoate trimer + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.46 dimer 2 * 42000, SDS-PAGE Flavobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.46 40
-
-
 Flavobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.46 2
-
 6-aminohexanoate trimer
-
 Flavobacterium sp.
3.5.1.46 2.4
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.46 8 9
-
 Flavobacterium sp.