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Literature summary extracted from

  • Fujiyama, K.; Zhang, Y.Z.; Negoro, S.; Urabe, I.; Okada, H.
    Characterization of hybrid enzymes between 6-aminohexanoate-dimer hydrolase and its analogues protein (1991), J. Ferment. Bioeng., 71, 298-302.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.46 expression in Escherichia coli Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.46 15
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Flavobacterium sp.
3.5.1.46 20
-
 N-(6-aminohexanoyl)-6-aminohexanoate for EII without mutation, substitution of Asp181 to Asn or Glu affects substrate binding Flavobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.46 intracellular
-
 Flavobacterium sp. 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.46 Flavobacterium sp.
-
-
-
3.5.1.46 Flavobacterium sp. KI72
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.46 to homogeneity, chromatography techniques, recombinant enzyme Flavobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.46 cell culture nylB gene, enzyme EII Flavobacterium sp.
-
3.5.1.46 cell culture nylB' gene, enzyme EII' Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.46 1.4
-
-
 Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.46 N-(4-aminobutyryl)-4-aminobutyric acid + H2O very low activity for EII Flavobacterium sp. 4-aminobutyric acid + 4-aminobutyric acid
-
 ?
3.5.1.46 N-(4-aminobutyryl)-4-aminobutyric acid + H2O very low activity for EII Flavobacterium sp. KI72 4-aminobutyric acid + 4-aminobutyric acid
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-4-aminobutyric acid + H2O low activity for EII Flavobacterium sp. 6-aminohexanoic acid + 4-aminobutyric acid
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-4-aminobutyric acid + H2O low activity for EII Flavobacterium sp. KI72 6-aminohexanoic acid + 4-aminobutyric acid
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O specific acitvity for EII' 0.5% that of EII Flavobacterium sp. 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O specific acitvity for EII' 0.5% that of EII Flavobacterium sp. KI72 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O 4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active Flavobacterium sp. 6-aminohexanoate + 8-aminooctanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O 4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active Flavobacterium sp. KI72 6-aminohexanoate + 8-aminooctanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-aniline + H2O highest activity for enzyme EII, EII' low activity Flavobacterium sp. 6-aminohexanoate + aniline
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-aniline + H2O highest activity for enzyme EII, EII' low activity Flavobacterium sp. KI72 6-aminohexanoate + aniline
-
 ?
3.5.1.46 N-(8-aminooctanoyl)-6-aminohexanoic acid + H2O low activity for enzyme EII, EII' no activity Flavobacterium sp. 8-aminooctanoic acid + 6-aminohexanoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.46 EII
-
 Flavobacterium sp.
3.5.1.46 EII'
-
 Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.46 50
-
 EII most heat-stable Flavobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.46 19
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.46 8.5 9.5 same value for enzymes EII, EII' Flavobacterium sp.