BRENDA - Enzyme Database

Preparation of bleomycinic acid: Hydrolysis of bleomycin B2 by a Fusarium acylagmatine amidohydrolase

Umezawa, H.; Takahashi, Y.; Fujii, A.; Saino, T.; Shirai, T.; Takita, T.; J. Antibiot. 26, 117-119 (1973)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.40
0.22
-
benzoyl agmatine
-
Fusarium anguioides
3.5.1.40
0.8
-
bleomycin B2
-
Fusarium anguioides
3.5.1.40
1.33
-
acetyl agmatine
-
Fusarium anguioides
3.5.1.40
2
-
propionyl agmatine
-
Fusarium anguioides
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.40
cytosol
-
Fusarium anguioides
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.40
Fusarium anguioides
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.40
partial
Fusarium anguioides
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.40
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.40
0.14
-
-
Fusarium anguioides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.40
acetyl agmatine + H2O
-
172038
Fusarium anguioides
acetic acid + agmatine
-
172038
Fusarium anguioides
?
3.5.1.40
benzoyl agmatine + H2O
-
172038
Fusarium anguioides
benzoic acid + agmatine
-
172038
Fusarium anguioides
?
3.5.1.40
bleomycin B2 + H2O
-
172038
Fusarium anguioides
bleomycinic acid + agmatine
-
172038
Fusarium anguioides
?
3.5.1.40
propionyl agmatine + H2O
-
172038
Fusarium anguioides
propionic acid + agmatine
-
172038
Fusarium anguioides
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.40
0.22
-
benzoyl agmatine
-
Fusarium anguioides
3.5.1.40
0.8
-
bleomycin B2
-
Fusarium anguioides
3.5.1.40
1.33
-
acetyl agmatine
-
Fusarium anguioides
3.5.1.40
2
-
propionyl agmatine
-
Fusarium anguioides
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.40
cytosol
-
Fusarium anguioides
5829
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.40
partial
Fusarium anguioides
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.40
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.40
0.14
-
-
Fusarium anguioides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.40
acetyl agmatine + H2O
-
172038
Fusarium anguioides
acetic acid + agmatine
-
172038
Fusarium anguioides
?
3.5.1.40
benzoyl agmatine + H2O
-
172038
Fusarium anguioides
benzoic acid + agmatine
-
172038
Fusarium anguioides
?
3.5.1.40
bleomycin B2 + H2O
-
172038
Fusarium anguioides
bleomycinic acid + agmatine
-
172038
Fusarium anguioides
?
3.5.1.40
propionyl agmatine + H2O
-
172038
Fusarium anguioides
propionic acid + agmatine
-
172038
Fusarium anguioides
?