BRENDA - Enzyme Database

Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides

Takahashi, Y.; Shirai, T.; Ishii, S.; J. Biochem. 77, 823-830 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.40
1,10-phenanthroline
84% inhibition at 50 mM
Fusarium anguioides
3.5.1.40
benzoyl-L-Arg-ethylester
Ki: 3.6 mM
Fusarium anguioides
3.5.1.40
benzoyl-L-Arg-NH2
Ki: 5 mM
Fusarium anguioides
3.5.1.40
Co2+
74% inhibition at 10 mM
Fusarium anguioides
3.5.1.40
Ni2+
85% inhibition at 10 mM
Fusarium anguioides
3.5.1.40
p-chloromercuribenzoic acid
77% inhibition at 0.1 mM, restored by addition of cysteine or 2-mercaptoethanol
Fusarium anguioides
3.5.1.40
Zn2+
95% inhibition at 10 mM
Fusarium anguioides
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.40
65000
-
gel filtration
Fusarium anguioides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.40
Fusarium anguioides
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.40
partial
Fusarium anguioides
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.40
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.1.40
2.7
-
-
Fusarium anguioides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.40
bleomycin B2 + H2O
specific with respect to the alkyl-chain length of the substrate
172037
Fusarium anguioides
bleomycinic acid + agmatine
-
172037
Fusarium anguioides
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.40
8
-
-
Fusarium anguioides
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.40
3.6
-
benzoyl-L-Arg-ethylester
-
Fusarium anguioides
3.5.1.40
5
-
benzoyl-L-Arg-NH2
-
Fusarium anguioides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.40
1,10-phenanthroline
84% inhibition at 50 mM
Fusarium anguioides
3.5.1.40
benzoyl-L-Arg-ethylester
Ki: 3.6 mM
Fusarium anguioides
3.5.1.40
benzoyl-L-Arg-NH2
Ki: 5 mM
Fusarium anguioides
3.5.1.40
Co2+
74% inhibition at 10 mM
Fusarium anguioides
3.5.1.40
Ni2+
85% inhibition at 10 mM
Fusarium anguioides
3.5.1.40
p-chloromercuribenzoic acid
77% inhibition at 0.1 mM, restored by addition of cysteine or 2-mercaptoethanol
Fusarium anguioides
3.5.1.40
Zn2+
95% inhibition at 10 mM
Fusarium anguioides
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.40
3.6
-
benzoyl-L-Arg-ethylester
-
Fusarium anguioides
3.5.1.40
5
-
benzoyl-L-Arg-NH2
-
Fusarium anguioides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.40
65000
-
gel filtration
Fusarium anguioides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.40
partial
Fusarium anguioides
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.40
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.1.40
2.7
-
-
Fusarium anguioides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.40
bleomycin B2 + H2O
specific with respect to the alkyl-chain length of the substrate
172037
Fusarium anguioides
bleomycinic acid + agmatine
-
172037
Fusarium anguioides
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.40
8
-
-
Fusarium anguioides