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Literature summary extracted from

  • Bernardo, K.; Hurwitz, R.; Zenk, T.; Desnick, R.J.; Ferlinz, K.; Schuchman, E.H.; Sandhoff, K.
    Purification, characterization, and biosynthesis of human acid ceramidase (1995), J. Biol. Chem., 270, 11098-11102.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 0.149
-
 N-lauroylsphingosine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 13000
-
 alpha,beta, 1 * 13000 + 1 * 40000, SDS-PAGE, treatment with beta-mercaptoethanol Homo sapiens
3.5.1.23 40000
-
 alpha,beta, 1 * 13000 + 1 * 40000, SDS-PAGE, treatment with beta-mercaptoethanol Homo sapiens
3.5.1.23 50000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 4454fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 0.00593
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 N-acetylsphingosine + H2O
-
 Homo sapiens acetic acid + sphingosine
-
 ?
3.5.1.23 N-hexanoylsphingosine + H2O
-
 Homo sapiens hexanoic acid + sphingosine
-
 ?
3.5.1.23 N-lauroylsphingosine + H2O
-
 Homo sapiens lauric acid + sphingosine
-
 ?
3.5.1.23 N-octanoylsphinganine + H2O
-
 Homo sapiens octanoic acid + sphingosine
-
 ?
3.5.1.23 N-octanoylsphingosine + H2O
-
 Homo sapiens octanoic acid + sphingosine
-
 ?
3.5.1.23 N-oleoylsphingosine + H2O
-
 Homo sapiens oleic acid + sphingosine
-
 ?
3.5.1.23 N-palmitoylsphinganine + H2O
-
 Homo sapiens palmitic acid + sphinganine
-
 ?
3.5.1.23 N-stearoylsphinganine + H2O
-
 Homo sapiens stearic acid + sphinganine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 dimer alpha,beta, 1 * 13000 + 1 * 40000, SDS-PAGE, treatment with beta-mercaptoethanol Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 3.8 4.3
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 3.5 4.6
-
 Homo sapiens