Literature summary extracted from

Bawab, S.E; Bielawska, A.; Hannun, Y.A.
Purification and characterization of a membrane-bound nonlysosomal ceramidase from rat brain (1999), J. Biol. Chem., 274, 27948-27955.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.5.1.23	phosphatidylglycerol	-	Rattus norvegicus
3.5.1.23	phosphatidylserine	-	Rattus norvegicus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.5.1.23	Mn2+	-	Rattus norvegicus
3.5.1.23	sphingosine	-	Rattus norvegicus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.1.23	90000	-	SDS-PAGE	Rattus norvegicus
3.5.1.23	95000	-	gel filtration	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.23	Rattus norvegicus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.23	22383fold	Rattus norvegicus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.5.1.23	brain	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.5.1.23	2.89	-	-	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.23	ceramide + H2O	C16-ceramide	Rattus norvegicus	sphingosine + fatty acid	-	?
3.5.1.23	dihydro-C16-ceramide + H2O	-	Rattus norvegicus	?	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5.1.23	7	10	-	Rattus norvegicus

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Release 2023.1 (January 2023)