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Literature summary extracted from

  • Gatt, S.
    Enzymatic hydrolysis of sphingolipids. I. Hydrolysis and synthesis of ceramides by an enzyme from rat brain (1966), J. Biol. Chem., 241, 3724-3730.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 sodium cholate
-
 Rattus norvegicus
3.5.1.23 Triton X-100
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 fatty acids
-
 Rattus norvegicus
3.5.1.23 laureate at pH 5 Rattus norvegicus
3.5.1.23 palmitate at pH 5 Rattus norvegicus
3.5.1.23 sphingosine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 0.2
-
 sphingosine
-
 Rattus norvegicus
3.5.1.23 0.3
-
 ceramide
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 100fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.23 -20°C, 1 year, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 ceramide + H2O
-
 Rattus norvegicus sphingosine + fatty acid
-
 r
3.5.1.23 N-oleyldihydro-sphingosine + H2O
-
 Rattus norvegicus ?
-
 r
3.5.1.23 N-palmitoyldihydrosphingosine + H2O
-
 Rattus norvegicus ?
-
 r
3.5.1.23 N-palmitoylsphingosine + H2O
-
 Rattus norvegicus palmitic acid + sphingosine
-
 r
3.5.1.23 N-stearoylsphingosine + H2O
-
 Rattus norvegicus stearic acid + sphingosine
-
 r
3.5.1.23 sphingosine + fatty acid
-
 Rattus norvegicus ceramide
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 4.8
-
-
 Rattus norvegicus