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Literature summary extracted from

  • Kalvero Airas, R.
    Kinetic studies on pantothenase of Pseudomonas fluorescens (1976), Biochem. J., 157, 415-421.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.22 oxalate
-
 Pseudomonas fluorescens
3.5.1.22 oxaloacetate noncompetitive inhibition, dependent on pH Pseudomonas fluorescens
3.5.1.22 Phenylmethanesulfonylfluoride
-
 Pseudomonas fluorescens
3.5.1.22 pyruvate
-
 Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.22 5
-
 pantothenic acid P-2, pH 7.5 at 28°C Pseudomonas fluorescens
3.5.1.22 7.6
-
 pantothenic acid pH-independent theoretical Km Pseudomonas fluorescens
3.5.1.22 12.5
-
 pantothenic acid 60 mM potassium phosphate, pH 7.4 Pseudomonas fluorescens
3.5.1.22 13
-
 pantothenic acid 20 mM potassium phosphate, pH 7.4 Pseudomonas fluorescens
3.5.1.22 15
-
 pantothenic acid 5 mM potassium phosphate, pH 7.4 Pseudomonas fluorescens
3.5.1.22 18
-
 pantothenic acid 10 mM sodium diphosphate/H2SO4, pH 7.5 Pseudomonas fluorescens
3.5.1.22 22
-
 pantothenic acid 10 mM Tris/H2SO4, pH 7.5 Pseudomonas fluorescens
3.5.1.22 24
-
 pantothenic acid 10 mM Tris/10 mM citric acid(KOH), pH 7.5 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.22 pantothenic acid + H2O Pseudomonas fluorescens
-
 ?
-
 ?
3.5.1.22 pantothenic acid + H2O Pseudomonas fluorescens P-2
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.22 Pseudomonas fluorescens
-
 P-2
-
3.5.1.22 Pseudomonas fluorescens
-
 NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water
-
3.5.1.22 Pseudomonas fluorescens P-2
-
 P-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.22
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens beta-alanine + D-pantoyl lactone + H2O
-
 r
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens P-2 beta-alanine + D-pantoyl lactone + H2O
-
 r
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens P-2 ?
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.22 5.5 9
-
 Pseudomonas fluorescens