BRENDA - Enzyme Database show

Pantothenase from Pseudomonas fluorescens

Kalvero Airas, R.; Methods Enzymol. 62, 267-275 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.22
oxalate
-
Pseudomonas fluorescens
3.5.1.22
oxaloacetate
noncompetitive inhibition, dependent on pH
Pseudomonas fluorescens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.22
7.6
-
pantothenic acid
pH-independent theoretical Km
Pseudomonas fluorescens
3.5.1.22
15
-
pantothenic acid
dependent on pH, phosphate buffer, pH 7.0, 20C
Pseudomonas fluorescens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.22
intracellular
-
Pseudomonas fluorescens
5622
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.22
50000
-
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
3.5.1.22
97400
-
gel filtration
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.22
pantothenic acid + H2O
Pseudomonas fluorescens
sole source of carbon and nitrogen
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.22
Pseudomonas fluorescens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.22
-
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.1.22
14.8
-
-
Pseudomonas fluorescens
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.22
-20C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years
Pseudomonas fluorescens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.22
pantothenate + H2O
-
171976
Pseudomonas fluorescens
2,4-dihydroxy-3,3-dimethylbutyric acid + beta-alanine
-
-
-
?
3.5.1.22
pantothenic acid + H2O
-
171976
Pseudomonas fluorescens
beta-alanine + D-pantoyl lactone
-
-
-
r
3.5.1.22
pantothenic acid + H2O
-
171976
Pseudomonas fluorescens
beta-alanine + D-pantoic acid
-
171976
Pseudomonas fluorescens
ir
3.5.1.22
pantothenic acid + H2O
sole source of carbon and nitrogen
171976
Pseudomonas fluorescens
?
-
-
-
-
3.5.1.22
pantoyl-gamma-aminobutyrate + H2O
-
171976
Pseudomonas fluorescens
4-aminobutyrate + D-pantoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.22
dimer
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.22
20
-
-
Pseudomonas fluorescens
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.1.22
30
35
above 30C rapidly inactivated, at 35C inactivation rate 7.2%/min
Pseudomonas fluorescens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.22
5.5
9.5
-
Pseudomonas fluorescens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.22
oxalate
-
Pseudomonas fluorescens
3.5.1.22
oxaloacetate
noncompetitive inhibition, dependent on pH
Pseudomonas fluorescens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.22
7.6
-
pantothenic acid
pH-independent theoretical Km
Pseudomonas fluorescens
3.5.1.22
15
-
pantothenic acid
dependent on pH, phosphate buffer, pH 7.0, 20C
Pseudomonas fluorescens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.22
intracellular
-
Pseudomonas fluorescens
5622
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.22
50000
-
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
3.5.1.22
97400
-
gel filtration
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.22
pantothenic acid + H2O
Pseudomonas fluorescens
sole source of carbon and nitrogen
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.22
-
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.1.22
14.8
-
-
Pseudomonas fluorescens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.22
-20C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.22
pantothenate + H2O
-
171976
Pseudomonas fluorescens
2,4-dihydroxy-3,3-dimethylbutyric acid + beta-alanine
-
-
-
?
3.5.1.22
pantothenic acid + H2O
-
171976
Pseudomonas fluorescens
beta-alanine + D-pantoyl lactone
-
-
-
r
3.5.1.22
pantothenic acid + H2O
-
171976
Pseudomonas fluorescens
beta-alanine + D-pantoic acid
-
171976
Pseudomonas fluorescens
ir
3.5.1.22
pantothenic acid + H2O
sole source of carbon and nitrogen
171976
Pseudomonas fluorescens
?
-
-
-
-
3.5.1.22
pantoyl-gamma-aminobutyrate + H2O
-
171976
Pseudomonas fluorescens
4-aminobutyrate + D-pantoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.22
dimer
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.22
20
-
-
Pseudomonas fluorescens
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.1.22
30
35
above 30C rapidly inactivated, at 35C inactivation rate 7.2%/min
Pseudomonas fluorescens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.22
5.5
9.5
-
Pseudomonas fluorescens