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Literature summary extracted from

  • Kalvero Airas, R.
    Pantothenase-based assay of pantothenic acid (1986), Methods Enzymol., 122, 33-35.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.22 nutrition vitamin determination, extremely sensitive assay of pantothenic acid in various dietary sources, as low as 5 nM concentration Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.22 Carbonate
-
Pseudomonas fluorescens
3.5.1.22 Cl-
-
Pseudomonas fluorescens
3.5.1.22 glycine
-
Pseudomonas fluorescens
3.5.1.22 oxaloacetate
-
Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.22 8
-
pantothenic acid
-
Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.22 pantothenic acid + H2O Pseudomonas fluorescens
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.22 Pseudomonas fluorescens
-
NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.22
-
Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
3.5.1.22 -20°C, 5 mM K2SO4, repeated cycles of freezing and thawing inactivate the enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.22 pantothenic acid + H2O
-
Pseudomonas fluorescens beta-alanine + D-pantoyl lactone + H2O
-
?
3.5.1.22 pantothenic acid + H2O
-
Pseudomonas fluorescens ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.22 25
-
-
Pseudomonas fluorescens