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Literature summary extracted from

  • Meinnel, T.; Schmitt, E.; Mechulam, Y.; Blanquet, S.
    Structural and biochemical characterization of the Escherichia coli arg gene product (1992), J. Bacteriol., 174, 2323-2331.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.16 plasmid pMC7 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.16 1.56
-
 acetylornithine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.16 42000
-
 pure NAO, SDS-PAGE Escherichia coli
3.5.1.16 42350
-
 calculated polypeptide, corresponding to AO nucleotide sequence Escherichia coli
3.5.1.16 52000
-
 SDS-PAGE Escherichia coli
3.5.1.16 62000
-
 gel filtration Escherichia coli
3.5.1.16 77000
-
 nondenaturing PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.16 N2-acety-L-ornithine + H2O Escherichia coli step in arginine biosynthesis L-ornithine + acetate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O Saccharomyces sp. step in arginine biosynthesis L-ornithine + acetate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O Escherichia coli 160-37 step in arginine biosynthesis L-ornithine + acetate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O Escherichia coli ATCC 9637 step in arginine biosynthesis L-ornithine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.16 Escherichia coli
-
 K-12
-
3.5.1.16 Escherichia coli
-
 K-37, UF121, UF121R, PAL123
-
3.5.1.16 Escherichia coli
-
 160-37
-
3.5.1.16 Escherichia coli
-
 ATCC9637
-
3.5.1.16 Escherichia coli 160-37
-
 160-37
-
3.5.1.16 Escherichia coli ATCC 9637
-
 ATCC9637
-
3.5.1.16 Saccharomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.16
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.16 87.1
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.16 N-acetyl-DL-methionine + H2O
-
 Escherichia coli methionine + acetate
-
 ?
3.5.1.16 N-acetyl-DL-methionine + H2O
-
 Saccharomyces sp. methionine + acetate
-
 ?
3.5.1.16 N-acetyl-DL-methionine + H2O
-
 Escherichia coli 160-37 methionine + acetate
-
 ?
3.5.1.16 N-acetyl-DL-methionine + H2O
-
 Escherichia coli ATCC 9637 methionine + acetate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli L-ornithine + acetate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O step in arginine biosynthesis Saccharomyces sp. L-ornithine + acetate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli 160-37 L-ornithine + acetate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli ATCC 9637 L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-ornithine + H2O
-
 Escherichia coli L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-ornithine + H2O
-
 Saccharomyces sp. L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-ornithine + H2O
-
 Escherichia coli 160-37 L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-ornithine + H2O
-
 Escherichia coli ATCC 9637 L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-benzoyl-L-ornithine + H2O
-
 Escherichia coli benzoate + ornithine
-
 ?
3.5.1.16 Nalpha-benzoyl-L-ornithine + H2O
-
 Escherichia coli 160-37 benzoate + ornithine
-
 ?
3.5.1.16 Nalpha-benzoyl-L-ornithine + H2O
-
 Escherichia coli ATCC 9637 benzoate + ornithine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.16 dimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.5.1.16 NAO
-
 Escherichia coli