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Literature summary extracted from

  • Hart, P.S.; Hymes, J.; Wolf, B.
    Isoforms of human serum biotinidase (1991), Clin. Chim. Acta, 197, 257-264.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.12 medicine plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.12 60000
-
 gel filtration, treated with N-glycanase Homo sapiens
3.5.1.12 71000
-
 gel filtration, treated with neuraminidase Homo sapiens
3.5.1.12 76500
-
 gel filtration, SDS-PAGE, normal serum biotinidase has 9 isoforms Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.12 biotin amide + H2O Homo sapiens cleaves D-biotinylamides and esters, recycling of the vitamin ?
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.12 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.12
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.12 blood serum, plasma, leukocytes Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.12 biotin amide + H2O cleaves D-biotinylamides and esters, recycling of the vitamin Homo sapiens ?
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
 Homo sapiens biotin + L-lysine
-
 ir
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
 Homo sapiens ?
-
 ?