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Literature summary extracted from

  • Ebrahim, H.; Dakshinamurti, K.
    A fluorometric assay for biotinidase (1986), Anal. Biochem., 154, 282-286.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.12 medicine plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.12 0.0077
-
 epsilon-N-biotinyl-L-lysine
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.12 biotin amide + H2O Homo sapiens
-
 ?
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.12 Homo sapiens
-
 human
-
3.5.1.12 no activity in Lactobacillus arabinosus
-
 ATCC 8014
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.12 blood serum, plasma, leukocytes Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.12 biotin amide + H2O
-
 Homo sapiens ?
-
 ?
3.5.1.12 biotinyl-6-aminoquinoline + H2O
-
 Homo sapiens biotin + 6-aminoquinoline
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
 Homo sapiens biotin + L-lysine
-
 ir
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
 Homo sapiens ?
-
 ?
3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O
-
 Homo sapiens biotin + p-aminobenzoic acid
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.12 4.5 6 with natural substrate biocytin Homo sapiens
3.5.1.12 6 7.5 with synthetic substrates Homo sapiens