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Literature summary extracted from
- Purification of biotinidase from human plasma and its activity on biotinyl peptides (1985), Biochemistry, 24, 2471-2476.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.12 | medicine | plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.12 | iodoacetamide | - |
Homo sapiens |  |
| 3.5.1.12 | phenylmethylsulfonyl fluoride | phenylmethanesulfonamide | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.12 | 0.005 | - |
epsilon-N-biotinyl-L-lysine | human serum | Homo sapiens | |
| 3.5.1.12 | 0.0062 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 3.5.1.12 | 0.00625 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 3.5.1.12 | 0.0065 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 76000 | - |
gel filtration | Homo sapiens |
| 3.5.1.12 | 78000 | - |
gel filtration | Homo sapiens |
| 3.5.1.12 | 115000 | - |
gel filtration, impure biotinidase | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | Enterococcus faecalis | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Homo sapiens | very short biotinyl peptides, enzyme is not active when biotin is attached to large native proteins, for example intact holocarboxylases | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Enterococcus faecalis | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | Enterococcus faecalis | - |
- |
- |
| 3.5.1.12 | Homo sapiens | - |
human | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.12 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | blood | serum, plasma, leukocytes | Homo sapiens | - |
| 3.5.1.12 | liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | very short biotinyl peptides, enzyme is not active when biotin is attached to large native proteins, for example intact holocarboxylases | Homo sapiens | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Enterococcus faecalis | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | PAB, artificial substrate | Homo sapiens | biotin + p-aminobenzoic acid | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.12 | monomer | 1 * 76000, SDS-PAGE | Homo sapiens |
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