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Literature summary extracted from

  • Bauer, K.; Kaufmann, W.; Ludwig, S.A.
    A simplified determination of penicillin amidase from E. coli (1971), Hoppe-Seyler's Z. Physiol. Chem., 352, 1723-1724.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.11 synthesis synthesis of 6-aminopenicillanate Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 penicillin G + H2O Escherichia coli
-
 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 4.6
-
 6-aminopenicillanic acid, pH 8, 27°C Escherichia coli
3.5.1.11 6.3
-
 6-aminopenicillanic acid, pH 7 Escherichia coli
3.5.1.11 6.6
-
 6-aminopenicillanic acid, pH 7.5 Escherichia coli
3.5.1.11 7.1
-
 6-aminopenicillanic acid, pH 8-8.5 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanate + phenylacetic acid
-
 r
3.5.1.11 penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanic acid + phenylacetic acid
-
 ?
3.5.1.11 phenylacetyl-L-asparagine + H2O
-
 Escherichia coli L-asparagine + phenylacetic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 7.2
-
-
 Escherichia coli