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Literature summary extracted from

  • Peterson, A.M.; Fernando, G.J.P.; Wells, M.A.
    Purification, characterization and cDNA sequence of an alkaline chymotrypsin from the midgut of Manduca sexta (1995), Insect Biochem. Mol. Biol., 25, 765-774.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.1
-
 Manduca sexta

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 chymostatin
-
 Manduca sexta
3.4.21.1 DFP
-
 Manduca sexta
3.4.21.1 PMSF
-
 Manduca sexta
3.4.21.1 TPCK
-
 Manduca sexta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.1 23600
-
 gel filtration Manduca sexta
3.4.21.1 24000
-
 x * 24000, SDS-PAGE Manduca sexta
3.4.21.1 24852
-
 x * 24852, calculation from nucleotide sequence Manduca sexta

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Manduca sexta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.1
-
 Manduca sexta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.1 midgut
-
 Manduca sexta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 additional information specific hydrolysis of peptide bonds on the carboxyl side of Tyr, Phe, Trp, His, Leu Thr and Gly Manduca sexta ?
-
 ?
3.4.21.1 N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide + H2O
-
 Manduca sexta N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.1 ? x * 24000, SDS-PAGE Manduca sexta
3.4.21.1 ? x * 24852, calculation from nucleotide sequence Manduca sexta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.1 10.5 11
-
 Manduca sexta