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Literature summary extracted from

  • Cornell, K.A.; Winter, R.W.; Tower, P.A.; Riscoe, M.K.
    Affinity purification of 5-methylthioadenosine kinase and 5-methylthioribose/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae (1996), Biochem. J., 317, 285-290.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.2.9 medicine potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels Escherichia coli
3.2.2.9 medicine potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.100 0.012
-
 5-methylthioribose pH 9.5, 37°C Klebsiella pneumoniae
3.2.2.9 0.0004
-
 5'-methylthioadenosine
-
 Escherichia coli
3.2.2.9 0.0004
-
 5'-methylthioadenosine
-
 Lupinus luteus
3.2.2.9 0.0087
-
 5'-methylthioadenosine
-
 Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.100 46000
-
 1 * 46000, SDS-PAGE Klebsiella pneumoniae
2.7.1.100 50000
-
 gel filtration Klebsiella pneumoniae
3.2.2.9 24000
-
 estimation from pfs gene product Klebsiella pneumoniae
3.2.2.9 26500
-
 SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.100 ATP + 5-methylthioribose Klebsiella pneumoniae
-
 ADP + 5-methylthioribose 1-phosphate
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Klebsiella aerogenes
-
 ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Klebsiella pneumoniae
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Klebsiella aerogenes
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Klebsiella pneumoniae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.100 Klebsiella pneumoniae
-
-
-
3.2.2.9 Escherichia coli
-
-
-
3.2.2.9 Klebsiella aerogenes
-
-
-
3.2.2.9 Klebsiella pneumoniae
-
 gram-negative pathogen, clinical isolate
-
3.2.2.9 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.100 ammonium sulfate, DEAE-Sepharose, 5-(p-aminophenyl)thioribose affinity chromatography Klebsiella pneumoniae
3.2.2.9
-
 Escherichia coli
3.2.2.9
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.100 ATP + 5-methylthioribose
-
 Klebsiella pneumoniae ADP + 5-methylthioribose 1-phosphate
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Lupinus luteus 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella pneumoniae 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella pneumoniae ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella pneumoniae adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.100 monomer 1 * 46000, SDS-PAGE Klebsiella pneumoniae
3.2.2.9 monomer 1 * 26500, SDS-PAGE Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
3.2.2.9 K. pneumoniae MTA/SAH nucleosidase
-
 Klebsiella pneumoniae